Changes in mitochondrial calcium concentration during the cardiac contraction cycle

• Published in: Cardiovascular research Vol. 27; no. 10; pp. 1800 - 1809
• Main Authors: Isenberg, Gerrit, Han, Song, Schiefer, Alfred, Wendt-Gallitelli, Maria-Fiora
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.10.1993
• Subjects: Animals; calcium; Calcium - analysis; Calcium - metabolism; Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacology; Cytosol - chemistry; Cytosol - drug effects; Cytosol - metabolism; Dinitrophenols - pharmacology; Electric Stimulation; Electron Probe Microanalysis; excitation-contraction coupling; Guinea Pigs; Mathematics; metabolic inhibition; mitochondria; Mitochondria, Heart - chemistry; Mitochondria, Heart - drug effects; Mitochondria, Heart - metabolism; Models, Cardiovascular; Myocardial Contraction - physiology; Myocardium - cytology
• ISSN: 0008-6363; 1755-3245
• DOI: 10.1093/cvr/27.10.1800

Objective: The aim was to examine whether mitochondrial Ca2+ fluxes are high enough to change mitochondrial and cytosolic calcium concentration during the contraction cycle. Methods: Isolated guinea pig ventricular myocytes were stimulated with paired voltage clamp pulses until contractions were maximal (2 mM [Ca2+]O, 36°C). At defined times of diastole or systole, the cells were shock frozen. Electronprobe microanalysis measured the concentration of total calcium in mitochondria (ΣCamito) and surrounding cytosol (ΣCac) Other experiments were performed to evaluate DNP sensitive mitochondrial Ca2+ uptake from depolarisation induced [Ca2+]c transients (K5indo-1 fluorescence). Results: At end of diastole, ΣCamito was 446 μmol·litre−1. During systole, ΣCamito increased with a 20 ms delay. A peak ΣCamito of 1050 μmol·litre−1 was measured 40 ms after start of systole, while 95 ms after start of systole ΣCamito had fallen to 530 μmol·litre−1. From the changes in ΣCamito the rates of net mitochondrial Ca2+ flux were estimated at 100 nmol·s−1·mg−1 protein for Ca2+ influx and 36 nmol·s−1·mg−1 protein for Ca2+ egress. Decay of ΣCamito was coupled to a rise in ΣNamito. ΣClmito and ΣKmito rose and fell in parallel with ΣCamito, suggesting Ca2+ activation of mitochondrial anion and cation channels. Activation of the non-specific permeability can be excluded. Block of mitochondrial Ca2+ uptake with DNP (100 μM) or FCCP (10 μM) increased the amplitude of the [Ca2+]c transients for 1-3 min by about 50%; evaluation of mitochondrial Ca2+ uptake from DNP sensitive difference signals, however, was hampered by sequestration of mitochondrial Ca2+ into the sarcoplasmic reticulum. Conclusions: Mitochondrial calcium content changes during each individual contraction cycle; a substantial amount of calcium is taken up during the systole and released during later systole and diastole. Cardiovascular Research 1993;27:1800-1809