Identification of mutations in porcine STAT5A that contributes to the transcription of CISH
Identification of causative genes or genetic variants associated with phenotype traits benefits the genetic improvement of animals. CISH plays a role in immunity and growth, however, the upstream transcriptional factors of porcine and the genetic variations in these factors remain unclear. In this s...
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Published in | Frontiers in veterinary science Vol. 9; p. 1090833 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
17.01.2023
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Subjects | |
Online Access | Get full text |
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Summary: | Identification of causative genes or genetic variants associated with phenotype traits benefits the genetic improvement of animals. CISH plays a role in immunity and growth, however, the upstream transcriptional factors of porcine
and the genetic variations in these factors remain unclear. In this study, we firstly identified the minimal core promoter of porcine
and confirmed the existence of STATx binding sites. Overexpression and RT-qPCR demonstrated STAT5A increased
transcriptional activity (
< 0.01) and mRNA expression (
< 0.01), while GATA1 inhibited
transcriptional activity (
< 0.01) and the following mRNA expression (
< 0.05 or
< 0.01). Then, the putative functional genetic variations of porcine
were screened and a PCR-SSCP was established for genotype g.508A>C and g.566C>T. Population genetic analysis showed the A allele frequency of g.508A>C and C allele frequency of g.566C>T was 0.61 and 0.94 in Min pigs, respectively, while these two alleles were fixed in the Landrace population. Statistical analysis showed that Min piglets with CC genotype at g.566C>T or Hap1: AC had higher 28-day body weight, 35-day body weight, and ADG than TC or Hap3: CT animals (
< 0.05,
< 0.05). Further luciferase activity assay demonstrated that the activity of g.508A>C in the C allele was lower than the A allele (
< 0.05). Collectively, the present study demonstrated that STAT5A positively regulated porcine
transcription, and SNP g.566C>T in the
was associated with the Min piglet growth trait. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Livestock Genomics, a section of the journal Frontiers in Veterinary Science Reviewed by: Lingyang Xu, Institute of Animal Sciences (CAAS), China; Xinyun Li, Huazhong Agricultural University, China Edited by: Yalan Yang, Agricultural Genomics Institute at Shenzhen (CAAS), China |
ISSN: | 2297-1769 2297-1769 |
DOI: | 10.3389/fvets.2022.1090833 |