Replicase-mediated resistance to potato virus Y in transgenic tobacco plants

• Published in: Molecular plant-microbe interactions Vol. 7; no. 1; pp. 15 - 22
• Main Authors: Audy, P, Palukaitis, P, Slack, S.A, Zaitlin, M
• Format: Journal Article
• Language: English
• Published: St Paul, MN APS Press 01.01.1994
• Subjects: Agronomy. Soil science and plant productions; Amino Acid Sequence; AMINO ACID SEQUENCES; Base Sequence; Biological and medical sciences; Cloning, Molecular; Defective Viruses - genetics; DISEASE RESISTANCE; DNA Primers; DNA-Directed DNA Polymerase - biosynthesis; DNA-Directed DNA Polymerase - genetics; Fundamental and applied biological sciences. Psychology; GENE; GENE TRANSFER; Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control; GENES; Genes, Viral; GENETIC TRANSFORMATION; GENETICA; GENETICS; GENETIQUE; Molecular Sequence Data; Nicotiana - genetics; Nicotiana - microbiology; NICOTIANA TABACUM; nuclear inclusion b protein; NUCLEOTIDE SEQUENCE; Phytopathology. Animal pests. Plant and forest protection; PLANT VIRUSES; Plant viruses and viroids; PLANTAS TRANSGENICAS; PLANTE TRANSGENIQUE; Plants, Genetically Modified; Plants, Toxic; Polymerase Chain Reaction; POTATO Y POTYVIRUS; Potyvirus - genetics; Potyvirus - pathogenicity; POTYVIRUS Y DE LA PATATA; POTYVIRUS Y POMME DE TERRE; PROTEINAS; PROTEINE; PROTEINS; REPLICATION; RESISTANCE AUX MALADIES; RESISTENCIA A LA ENFERMEDAD; Restriction Mapping; RNA POLYMERASE; SECUENCIA NUCLEICA; Sequence Deletion; SEQUENCE NUCLEIQUE; STRUCTURAL GENES; TRANSFERASAS; TRANSFERASE; TRANSFERASES; TRANSFERENCIA DE GENES; TRANSFERT DE GENE; TRANSFORMACION GENETICA; TRANSFORMATION GENETIQUE; TRANSGENIC PLANTS; VIRUS DE LAS PLANTAS; VIRUS DES VEGETAUX; Genome organization; Genetic control; Plant pathogen; Nucleotide sequence; Enzyme; Transferases; Nicotiana tabacum; RNA-directed RNA polymerase; Virus; Resistance; Nucleotidyltransferases; Potato virus Y; Dicotyledones; Angiospermae; Spermatophyta; Transgenic plant; Potyvirus; Solanaceae; Aminoacid sequence
• ISSN: 0894-0282; 1943-7706
• DOI: 10.1094/mpmi-7-0015

Nicotiana tabacum 'Turkish Samsun NN' plants were transformed with nuclear inclusion b (NIb) gene sequences of potato virus Y, O strain (PVYO). The full-length construct included an additional in-frame initiation codon contiguous to the putative N-terminal amino acid codon and a stop codon replacing the C-terminal amino acid codon. Of 13 independently transformed lines, four yielded 37 (out of 100) plants in the R1 generation that were resistant to PVYO infection. Progeny of 13 out of 15 of R1 plants tested expressed resistance in the R2 generation. Conversely, 30 independently transformed tobacco lines expressing essentially the same sequence but deleted for the Gly-Asp-Asp (GDD) motif were not resistant. Two other constructs encoding either the 5'-deleted or 3'-truncated NIb gene, but harboring the GDD motif, conferred resistance to PVYO in some tobacco plants. Despite the high level of nucleotide and amino acid identity shared by strains PVYO and PVYN for the NIb gene, PVYN replication was found in all PVYO-resistant plants. However, plants of one R2 line showed reduced PVYN replication.