Simultaneous detection of mitochondrial viscosity and peroxynitrite in livers from subjects with drug-induced fatty liver disease using a novel fluorescent probe

• Published in: Talanta (Oxford) Vol. 260; p. 124591
• Main Authors: Niu, Linqiang, Cao, Qijuan, Zhang, Tian, Zhang, Yahong, Liang, Tingting, Wang, Jianhong
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2023
• Subjects: Animals; Chemical and Drug Induced Liver Injury; Drug-induced fatty liver disease; Dual responsive; electron transport chain; fatty liver; fluorescence; fluorescent dyes; Fluorescent Dyes - pharmacology; Fluorescent probe; liver; Mice; Mitochondria; Peroxynitrite; Peroxynitrous Acid; reactive oxygen species; Viscosity; Viscosity; Peroxynitrite; Drug-induced fatty liver disease; Dual responsive; Fluorescent probe
• ISSN: 0039-9140; 1873-3573; 1873-3573
• DOI: 10.1016/j.talanta.2023.124591

Drug-induced fatty liver disease (DIFLD) is a basic clinicopathological example of drug-induced liver injury (DILI). Some drugs can inhibit β-oxidation in hepatocyte mitochondria, leading to steatosis in the liver. Additionally, drug-induced inhibition of β-oxidation and the electron transport chain (ETC) can lead to increased production of reactive oxygen species (ROS) such as peroxynitrite (ONOO−). Therefore, it is reasonable to suspect that compared to a healthy liver, viscosity and ONOO− levels are elevated in livers during DIFLD. A novel, smart, dual-response fluorescent probe—Mito-VO—was designed and synthesized for the simultaneous detection of viscosity and ONOO− content. This probe had a large emission shift of 293 nm and was capable of monitoring the viscosity of, and the ONOO− content in, cell and animal models alike, either individually or simultaneously. For the first time, Mito-VO was successfully used to demonstrate the elevated viscosity and the amount of ONOO− in livers from mice with DIFLD. [Display omitted] •A dual-response fluorescent probe could simultaneously detect viscosity and ONOO−.•The probe had a large emission shift of 293 nm.•The probe was capable of monitoring cell and animal models alike.•The probe was used to detect viscosity and ONOO− in livers from mice with DIFLD.