Search for bovine papilloma virus DNA in bovine ocular squamous cell carcinomas (BOSCC) and BOSCC-derived cell lines

• Published in: American journal of veterinary research Vol. 53; no. 9; p. 1477
• Main Authors: Rutten, V.P.M.G. (University of Utrecht, Utrecht, Netherlands), Klein, W.R, Jong, M.A.C. de, Quint, W, Otter, W. den, Ruitenberg, E.J, Melchers, W.J.G
• Format: Journal Article
• Language: English
• Published: United States 01.09.1992
• Subjects: ADN; Animals; Biopsy - veterinary; Blotting, Southern; BOVIN; BOVINE PAPILLOMAVIRUS; Bovine papillomavirus 1 - genetics; Bovine papillomavirus 1 - isolation & purification; CARCINOMA; Carcinoma, Squamous Cell - microbiology; Carcinoma, Squamous Cell - veterinary; CARCINOME; CATTLE; Cattle Diseases - microbiology; CELL CULTURE; CULTIVO DE CELULAS; CULTURE DE CELLULE; DETECTION; DNA; DNA Probes; DNA, Viral - analysis; Electrophoresis, Agar Gel; Eye Neoplasms - microbiology; Eye Neoplasms - veterinary; Female; GANADO BOVINO; Nucleic Acid Hybridization; PAPILLOMAVIRUS BOVIN; Tumor Cells, Cultured; VIRUS DEL PAPILOMA BOVINO
• ISSN: 0002-9645; 1943-5681
• DOI: 10.2460/ajvr.1992.53.09.1477

Although the cause of bovine ocular squamous cell carcinoma (BOSCC) is attributed to viruses in addition to cofactors (eg, UV light), to our knowledge, the final causative agent has not been described. Bovine papilloma virus (BPV)-like particles were detected in approximately 33% of various putative precursor lesions of BOSCC. In contrast, it was reported that, using BPV-specific antibodies, it was not possible to detect viral antigens in BOSCC. Fourteen established BOSCC and 9 BOSCC-derived cell lines were examined for BPV DNA. Probes of all 6 known BPV types were used in various hybridization assays. Neither Southern blot analysis, under high and low stringency conditions, nor in situ hybridization resulted in detection of BPV DNA. Papilloma viruses were not observed in electron microscopic studies. Results exclude direct association between BOSCC and BPV types 1 to 6, or as yet unknown closely related BPV types. However, BPV may contribute to induction of precursor lesions or events leading to carcinogenic transformation, without being relevant for maintenance of the tumor.