Novel GUS expression patterns following transposition of an enhancer trap Ds element in Arabidopsis

• Published in: Molecular & general genetics Vol. 249; no. 4; p. 357
• Main Authors: Klimyuk, V.I, Nussaume, L, Harrison, K, Jones, J.D.G. (John Innes Ins., Norwich (United Kingdom). Sainsbury Lab.)
• Format: Journal Article
• Language: English
• Published: Germany 10.12.1995
• Subjects: Arabidopsis - genetics; ARABIDOPSIS THALIANA; Base Sequence; Blotting, Southern; DNA Transposable Elements - genetics; Enhancer Elements, Genetic - genetics; ENZIMAS; ENZYME; EXPRESION GENICA; EXPRESSION DES GENES; GENE; Gene Expression Regulation, Plant - genetics; GENES; Genes, Reporter - genetics; Glucuronidase - genetics; Glucuronidase - metabolism; Histocytochemistry; Microscopy, Phase-Contrast; Molecular Sequence Data; Mutation - genetics; Phenotype; Polymerase Chain Reaction; TATA Box - genetics; Transcriptional Activation; Transformation, Genetic - genetics; TRANSPOSICION DE GENES; TRANSPOSITION DE GENES
• ISSN: 0026-8925; 1432-1874
• DOI: 10.1007/bf00287097

Enhancer trap derivatives of the maize Dissociation (Ds) transposon were introduced into Arabidopsis thaliana. The enhancer trap Ds was so designed that upon transposition to sites containing regulatory sequences in adjacent genomic DNA, transcription of a Ds-borne beta-glucuronidase (GUS) gene would be activated. Sixty percent of all transposition events were associated with GUS expression patterns including one linked to a mutant phenotype. Patterns of GUS expression were found in various organs and were stably inheritable in the F4 and F5 progenies. These results demonstrate the potential value of the technique as a means for detection of developmentally regulated genes and analysis of their function. The enhancer trap construct used in the experiments, as well as the seeds of primary transformants are publicly available.