Novel GUS expression patterns following transposition of an enhancer trap Ds element in Arabidopsis
Enhancer trap derivatives of the maize Dissociation (Ds) transposon were introduced into Arabidopsis thaliana. The enhancer trap Ds was so designed that upon transposition to sites containing regulatory sequences in adjacent genomic DNA, transcription of a Ds-borne beta-glucuronidase (GUS) gene woul...
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Published in | Molecular & general genetics Vol. 249; no. 4; p. 357 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Germany
10.12.1995
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Subjects | |
Online Access | Get more information |
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Summary: | Enhancer trap derivatives of the maize Dissociation (Ds) transposon were introduced into Arabidopsis thaliana. The enhancer trap Ds was so designed that upon transposition to sites containing regulatory sequences in adjacent genomic DNA, transcription of a Ds-borne beta-glucuronidase (GUS) gene would be activated. Sixty percent of all transposition events were associated with GUS expression patterns including one linked to a mutant phenotype. Patterns of GUS expression were found in various organs and were stably inheritable in the F4 and F5 progenies. These results demonstrate the potential value of the technique as a means for detection of developmentally regulated genes and analysis of their function. The enhancer trap construct used in the experiments, as well as the seeds of primary transformants are publicly available. |
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Bibliography: | F30 97B6727 |
ISSN: | 0026-8925 1432-1874 |
DOI: | 10.1007/bf00287097 |