Use of Aspergillus fumigatus mycelial antigens in enzyme-linked immunosorbent assay and counter-immunoelectrophoresis

• Published in: Journal of medical microbiology Vol. 16; no. 1; pp. 97 - 105
• Main Authors: WILSON, ELAINE V, HEARN, VERONICA M
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01.02.1983
• Subjects: Antigens, Fungal - immunology; Aspergillosis - immunology; Aspergillus fumigatus; Aspergillus fumigatus - immunology; Counterimmunoelectrophoresis; Enzyme-Linked Immunosorbent Assay; Humans; Immune Sera; Immunoelectrophoresis; Immunoenzyme Techniques; Immunoglobulin G - immunology
• ISSN: 0022-2615; 1473-5644
• DOI: 10.1099/00222615-16-1-97

1 Mycological Reference Laboratory, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT Received March 12, 1982 Accepted May 17, 1982 SUMMARY. Antigens obtained from the ruptured mycelium of Aspergillus fumigatus were separated on the basis of their attachment or non-attachment to Concanavalin A-linked Sepharose gel. They were analysed by double-diffusion and two-dimensional immunoelectrophoresis with rabbit antiserum and sera from patients with aspergillus-related disease. Their sensitivity was assessed in an enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G. Sera which gave very low or high extinction readings on ELISA gave, in general, comparable results on counter-immunoelectrophoresis; but weak electrophoretic reactions were not closely paralleled by ELISA results. The unfractionated extract and the fraction bound by Concanavalin A-Sepharose had similar ability to detect specific IgG in sera from patients with aspergillosis.