Antizyme-Dependent and -Independent Mechanisms Are Responsible for Increased Spermidine Transport in Amino Acid-Restricted Human Cancer Cells

Amino acid deprivation can inhibit tumour cell proliferation. Since polyamines are required for cell growth, we hypothesised that their regulatory pathways can respond to amino acid restriction. We report here that exposure of human colon adenocarcinoma Caco-2 cells to a medium restricted for a sing...

Full description

Saved in:
Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 256; no. 3; pp. 646 - 651
Main Authors Aubel, C., Chabanon, H., Persson, L., Thiman, L., Ferrara, M., Brachet, P.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 24.03.1999
Elsevier
Subjects
amino acid restriction
Amino Acids - metabolism
Amino Acids - pharmacology
Biochemistry, Molecular Biology
Biological Transport - drug effects
Caco-2 Cells
Culture Media
Cycloheximide - pharmacology
Glucose - metabolism
Humans
Kinetics
Life Sciences
neoplasms
nutrient intake
Polyamines - metabolism
Protein Biosynthesis
Protein Synthesis Inhibitors - pharmacology
Proteins - genetics
Proteins - metabolism
RNA, Messenger - metabolism
Spermidine - metabolism
CANCER
Online AccessGet full text

Cover

More Information
Summary:Amino acid deprivation can inhibit tumour cell proliferation. Since polyamines are required for cell growth, we hypothesised that their regulatory pathways can respond to amino acid restriction. We report here that exposure of human colon adenocarcinoma Caco-2 cells to a medium restricted for a single amino acid, but not for D-glucose, activates spermidine transport. The increase was rapid and seemed transient with a maximum 4-6 hr after amino acid removal. Kinetics showed that the maximal velocity of transport was solely increased in L-methionine- or L-leucine-deprived cells, indicating increased number of transporters. The intracellular level of complex of ornithine decarboxylase (ODC) with antizyme, a negative regulator of polyamine transport, was decreased by 16-29% in amino acid-deprived cells. However, exposure to limited amounts of amino acid increased transport without altering the ODC-antizyme complex level. We propose that antizyme-independent mechanisms, sensitive to the amino acid concentration, also participate to the control of spermidine transport.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1999.0397