Stacked optical waveguide photobioreactor for high density algal cultures

• Published in: Bioresource technology Vol. 171; pp. 495 - 499
• Main Authors: Jung, Erica E., Jain, Aadhar, Voulis, Nina, Doud, Devin F.R., Angenent, Largus T., Erickson, David
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.11.2014; Elsevier
• Subjects: Algal-based biorefineries; Biofuel; Biofuel production; Biological and medical sciences; Biomass; Biotechnology; Cell Culture Techniques - instrumentation; Energy; Ethylenes - biosynthesis; Fundamental and applied biological sciences. Psychology; Genetic Engineering - methods; Industrial applications and implications. Economical aspects; Microalgae - genetics; Microalgae - growth & development; Microalgae cultivation; Optical waveguides; Photobioreactor; Photobioreactors; Microalgae cultivation; Biofuel; Algal-based biorefineries; Photobioreactor; Optical waveguides; High density; Biorefinery; Alga; Microorganism; Waveguide; Culture; Algal culture
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2014.08.093

•An ultracompact photobioreactor with planar scattering light waveguides was developed.•Alleviates the problem of non uniform light distribution in traditional bioreactors.•Eightfold increase in algal biomass accumulation over a control without waveguides.•The photobioreactor supported consistent production of ethylene over 45days.•Twofold volumetric productivity increase over a conventional flat plate bioreactor. In this work, an ultracompact algal photobioreactor that alleviates the problem of non-optimal light distribution in current algae photobioreactor systems, by incorporating stacked layers of slab waveguides with embedded light scatterers, is presented. Poor light distribution in traditional photobioreactor systems, due to self-shading effects, is responsible for relatively low volumetric productivity. The optimal conditions for operating a 10-layer bioreactor are outlined. The bioreactor exhibits the ability to sustain uniform biomass growth throughout the bioreactor for 3weeks, and demonstrates an 8-fold increase in biomass productivity. Using a genetically engineered algal strain, constant secreted ethylene production for over 45days is also demonstrated. Since the stacked architecture leads to improved light distribution throughout the volume of the bioreactor, it reduces the need for culture mixing for optimum light distribution, and thereby potentially reducing operational costs.