Pituitary steroids in two teleost species: Immunohistological and biochemical studies
Antisera raised against steroid hormones [estradiol-17β (E 2), testosterone (T), 11β-hydroxyandrostenedione (OHA)] were used to localize immunoreactive material in fixed and paraffin-embedded pituitaries of the African catfish and the rainbow trout. Organic extracts of pituitary homogenates were ana...
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Published in | General and comparative endocrinology Vol. 76; no. 1; pp. 63 - 72 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
San Diego, CA
Elsevier Inc
01.10.1989
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Antisera raised against steroid hormones [estradiol-17β (E
2), testosterone (T), 11β-hydroxyandrostenedione (OHA)] were used to localize immunoreactive material in fixed and paraffin-embedded pituitaries of the African catfish and the rainbow trout. Organic extracts of pituitary homogenates were analyzed for steroid hormones by radioimmunoassay and gas chromatography-mass spectrometry (E
2 in female catfish only). With the exception of an E
2-positive cell type in the catfish neurointermediate lobe, steroid immunoreactivity was found to be restricted to the cytoplasm of adenohypophyseal cells, which were also labeled after incubation with catfish α,β-gonadotropin and salmon gonadotropin antisera, respectively. Steroid levels determined by radioimmunoassay in the catfish ranged between 85 and 628 pg/pituitary, while lower levels (2–8 pg/pituitary) were found in the rainbow trout. E
2 was identified by gas chromatography-mass spectrometry at a level of 84 pg/pituitary. The observation that immunolabeling after steroid antiserum incubation is confined mainly to gonadotrops provides morphological evidence for direct steroid effects on this particular cell type. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0016-6480 1095-6840 |
DOI: | 10.1016/0016-6480(89)90033-6 |