Study of the genus Torreya (Taxaceae) based on chloroplast genomes

• Published in: Frontiers in bioscience (Landmark. Print) Vol. 27; no. 1; p. 9
• Main Authors: Miao, Zhen-Peng, Niu, Xiang-Na, Wang, Rong-Bin, Huang, Long, Ma, Bei-Bei, Li, Jian-Hui, Hong, Xin
• Format: Journal Article
• Language: English
• Published: Singapore IMR Press 10.01.2022
• Subjects: Base Sequence; chloroplast genome; Genome, Chloroplast - genetics; molecular ecology; Phylogeny; single-copy orthologous genes; Taxaceae; torreya; Molecular ecology; Phylogeny; Single-copy orthologous genes; Chloroplast genome
• ISSN: 2768-6701; 2768-6698
• DOI: 10.31083/j.fbl2701009

Species of the genus are similar in morphology, and their morphological taxonomic characteristics are not stable because of environmentally induced changes. Therefore, morphology is insufficient for understanding their relationships. Chloroplast genome sequencing technology provides a powerful tool for molecular analysis to get more infomation for classification and identification of genus. A total of 4 chloroplast genome of , including . , . , . var. and . var. , were sequenced and annotated. Campartive genome and phylogenetic tree were provided for variation analysis. The chloroplast genome size of the four samples is about 137 kb, the inverted repeat (IR) regions are identified in the genus . Genome comparison using mVISTA showed high sequence similarity among different species. Regions with divergence in exon regions include , , , , , , , , , , and . The phylogenetic tree based on 73 single-copy genes showed a clearer relationships among different species of . All genomes of the four species consist of two short IR regions, and results of the phylogenetic analysis concluded that should be considered as var. or treated as a sister species. . var. should be treated as a variety of . according to molecular evidence, supporting the originally published proposal.