Analyzing the biosensor signal in flows: Studies with glucose optrodes

• Published in: Talanta (Oxford) Vol. 131; pp. 74 - 80
• Main Authors: Kivirand, K., Floren, A., Kagan, M., Avarmaa, T., Rinken, T., Jaaniso, R.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2015
• Subjects: Biocatalysis; Biosensing Techniques; Biosensors; Calibration; Channels; Design analysis; Design engineering; Dual-optrode biosensor; Fiber Optic Technology; Flow regime; Glucose; Glucose - analysis; Glucose - metabolism; Glucose Oxidase - metabolism; Mathematical models; Modeling; Models, Theoretical; Oxygen - metabolism; Pre-steady state calibration; Slopes; Pre-steady state calibration; Flow regime; Glucose; Modeling; Dual-optrode biosensor
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2014.07.061

Responses of enzymatic bio-optrodes in flow regime were studied and an original model was proposed with the aim of establishing a reliable method for a quick determination of biosensor signal parameters, applicable for biosensor calibration. A dual-optrode glucose biosensor, comprising of a glucose bio-optrode and a reference oxygen optrode, both placed into identical flow channels, was developed and used as a model system. The signal parameters of this biosensor at different substrate concentrations were not dependent on the speed of the probe flow and could be determined from the initial part of the biosensor transient phase signal, providing a valuable tool for rapid analysis. In addition, the model helped to design the biosensor system with reduced impact of enzyme inactivation to the system stability (20% decrease of the enzyme activity lead to only a 1% decrease of the slope of the calibration curve) and hence significantly prolong the effective lifetime of bio-optrodes. [Display omitted] •Dual-optrode flow cell biosensor is constructed and optimized for stable output.•Analytical model is developed for sensing with two-substrate reaction in flow.•Method is given for quick pre-steady state determination of analyte concentration.