Enhancement of the horseradish peroxidase-catalyzed chemiluminescent oxidation of cyclic diacyl hydrazides by 6-hydroxybenzothiazoles

• Published in: Analytical biochemistry Vol. 145; no. 1; pp. 96 - 100
• Main Authors: Thorpe, Gary H.G., Kricka, Larry J., Gillespie, Eileen, Moseley, Susan, Amess, Robert, Baggett, Neil, Whitehead, Thomas P.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 15.02.1985; Elsevier
• Subjects: 6-hydroxybenzothiazoles; Analytical biochemistry: general aspects, technics, instrumentation; Analytical, structural and metabolic biochemistry; Armoracia lapathifolia; Biological and medical sciences; Catalysis; chemiluminescence; enhanced chemiluminescence; firefly luciferin; Fundamental and applied biological sciences. Psychology; Horseradish Peroxidase - metabolism; horseradish peroxidase conjugates; Hydrazines - chemical synthesis; Hydrogen-Ion Concentration; Indicators and Reagents - chemical synthesis; isoluminol; Luminescent Measurements; luminol; Oxidation-Reduction; peroxidase; Peroxidases - metabolism; Spectrometry, Fluorescence; Thiazoles - chemical synthesis; 6-hydroxybenzothiazoles; enhanced chemiluminescence; horseradish peroxidase conjugates; firefly luciferin; luminol; Immunoperoxidase technique; Immunological method
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/0003-2697(85)90332-X

6-Hydroxybenzothiazole, 2-cyano-6-hydroxybenzothiazole, and 2-(6-hydroxy-2-benzothiazolyl)thiazole-4-carboxylic acid (dehydroluciferin) dramatically enhance light emission from the horseradish peroxidase conjugate catalyzed oxidation of luminol, isoluminol, N-(6-aminobutyl)- N-ethyl isoluminol, and 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide by either peroxide or perborate. Light emission is enhanced by up to 1000-fold, which is an improvement over the enhancement previously observed using firefly luciferin (4,5-dihydro-2-(6-hydroxy-2-benzothiazolyl)thiazole-4-carboxylic acid). Enhancement is influenced by enhancer concentration and pH. Spectral scans of light emitted in enhanced and unenhanced reactions are similar, suggesting that aminophthalate products, and not the enhancers, are the emitters.