Sequential expression of proto-oncogenes during a mouse erythroleukemia cell differentiation

• Published in: Biochemical and biophysical research communications Vol. 135; no. 3; pp. 1112 - 1118
• Main Authors: Todokoro, Kazuo, Ikawa, Yoji
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 28.03.1986; Elsevier
• Subjects: Animals; Applied sciences; Cell Differentiation - drug effects; Cell Division; Dimethyl Sulfoxide - pharmacology; Exact sciences and technology; Gene Expression Regulation; Globins - genetics; Leukemia, Erythroblastic, Acute - genetics; Leukemia, Erythroblastic, Acute - pathology; Mice; Other techniques and industries; Proto-Oncogenes; Transcription, Genetic
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(86)91043-0

To investigate the possible involvement of proto-oncogenes in the regulation of cell differentiation and proliferation, their transcriptional levels were measured by Northern blot analysis during the process of dimethyl sulfoxide-induced differentiation of mouse erythroleukemia cells. The differentiation into erythrocytes was accompanied by sequential transient expression of the proto-oncogenes in the order of c- fos , c- myb , c- myc and c-K- ras , during the first 48 hr of differentiation induction. Following the transient expression of these proto-oncogenes, adult type β S-globin and embryonic type y 1 - globin gene transcripts appeared and accumulated in terminally differentiated cells. The expression of ten other proto-oncogenes examined here were not detected, nor dramatically changed during differentiation process.