Transfection and structural properties of phytanyl substituted gemini surfactant-based vectors for gene delivery
In this study, the transfection ability and cytotoxicity of a series of phytanyl substituted gemini surfactants, rationally designed and synthesized in an attempt to create cationic surfactants that will improve transfection efficiencies of non-viral vectors was evaluated in OVCAR-3 cells at the cha...
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Published in | Physical chemistry chemical physics : PCCP Vol. 15; no. 47; pp. 251 - 2516 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Cambridge
Royal Society of Chemistry
21.12.2013
|
Subjects | |
Online Access | Get full text |
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Summary: | In this study, the transfection ability and cytotoxicity of a series of phytanyl substituted gemini surfactants, rationally designed and synthesized in an attempt to create cationic surfactants that will improve transfection efficiencies of non-viral vectors was evaluated in OVCAR-3 cells at the charge ratios (N
+
/P
−
) of 2 : 1, 5 : 1, and 10 : 1. Particle sizes, zeta potentials, and small angle X-ray scattering (SAXS) profiles were also determined. For each gemini surfactant complex, the transfection efficiency and cytotoxicity are observed to go through a more or less well-evidenced maximum, occurring at different values of the charge ratio (N
+
/P
−
), depending on the surfactant structure. Considering both results of
in vitro
transfection efficiency and cytotoxicity, the optimal charge ratio to formulate the complexes containing phy-3-
m
was found to be 5 : 1. The particle size decreased, while zeta potential increased with increasing N
+
/P
−
. Comparing particle size and zeta potential with transfection efficiency, no correlation between size/zeta potential and transfection ability was observed. Analysis of SAXS profiles indicates that the ability of phy-3-
m
delivery system to adopt multiple phases correlated well with their higher transfection efficiency in OVCAR-3 cells.
Higher transfection efficiency achieved with the phy-3-
m
-based delivery systems in OVCAR-3 cells appears correlated with their ability to adopt multiple phases. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1463-9076 1463-9084 |
DOI: | 10.1039/c3cp52621f |