Secondary Metabolites from Fungal Endophytes of Echinacea purpurea Suppress Cytokine Secretion by Macrophage-Type Cells

Botanical extracts of Echinacea purpurea have been widely used for the treatment of upper respiratory infections. We sought to chemically examine fungal endophytes inhabiting E. purpurea, and to identify compounds produced by these endophytes with in vitro cytokine-suppressive activity. Twelve isola...

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Published inNatural product communications Vol. 11; no. 8; pp. 1143 - 1146
Main Authors Kaur, Amninder, Oberhofer, Martina, Juzumaite, Monika, Raja, Huzefa A., Gulledge, Travis V., Kao, Diana, Faeth, Stanley H., Laster, Scott M., Oberlies, Nicholas H., Cech, Nadja B.
Format Journal Article
LanguageEnglish
Published Los Angeles, CA SAGE Publications 01.08.2016
Subjects
Animals
Echinacea
Endophytes
Macrophages
Mice
Plant Extracts
Tumor Necrosis Factor-alpha
Fungal endophytes
Inflammation
Seeds
Echinacea purpurea
TNF-α
Asteraceae
inflammation
seeds
fungal endophytes
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Summary:Botanical extracts of Echinacea purpurea have been widely used for the treatment of upper respiratory infections. We sought to chemically examine fungal endophytes inhabiting E. purpurea, and to identify compounds produced by these endophytes with in vitro cytokine-suppressive activity. Twelve isolates from surface sterilized seeds of E. purpurea were subjected to fractionation and major components were isolated. Sixteen secondary metabolites belonging to different structural classes were identified from these isolates based on NMR and mass spectrometry data. The compounds were tested for their influence on cytokine secretion by murine macrophage-type cells. Alternariol (1), O-prenylporriolide (4), porritoxin (10) β-zearalenol (13), and (S)-zearalenone (14) inhibited production of TNF-α from RAW 264.7 macrophages stimulated with LPS in the absence of any significant cytotoxicity. This is the first report of a cytokine-suppressive effect for 4. The results of this study are particularly interesting given that they show the presence of compounds with cytokine-suppressive activity in endophytes from a botanical used to treat inflammation. Future investigations into the role of fungal endophytes in the biological activity of E. purpurea dietary supplements may be warranted.
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ISSN:1934-578X
1555-9475
DOI:10.1177/1934578X1601100827