Gene organization and sequence determination of the two botulinum neurotoxin gene clusters in Clostridium botulinum type A(B) strain NCTC 2916

The gene organization and nucleotide sequence of the type A and B BoNT-gene clusters in Clostridium botulinum strain NCTC 2916 were studied. The aim was to clarify the organization of genes within C. botulinum type A strains possessing an unexpressed BoNT/B gene. The BoNT/A-gene cluster includes gen...

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Bibliographic Details
Published inCurrent microbiology Vol. 36; no. 4; pp. 226 - 231
Main Authors Jovita, M.R. (Institute of Food Research, Early Gate, Reading, Berkshire, UK.), Collins, M.D, East, A.K
Format Journal Article
LanguageEnglish
Published New York, NY Springer 01.04.1998
Springer Nature B.V
Subjects
Amino acids
Bacteriology
Biological and medical sciences
Botulinum Toxins - genetics
Botulinum Toxins, Type A - genetics
Botulism
CLOSTRIDIUM BOTULINUM
Clostridium botulinum - genetics
DNA, Bacterial - analysis
Fundamental and applied biological sciences. Psychology
GENBANK/Y14238
GENBANK/Y14239
Genes
Genes, Bacterial - genetics
Genetics
Microbiology
MOLECULAR SEQUENCE DATA
Multigene Family - genetics
Neurotoxins
Polymerase Chain Reaction
Gene cluster
Clostridium botulinum
Nucleotide sequence
Enzyme
Toxilysin
Clostridiales
Metalloendopeptidases
Gene organization
Peptidases
Toxin
Clostridiaceae
Neurotoxin
Bacteria
Hydrolases
Structural analysis
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Summary:The gene organization and nucleotide sequence of the type A and B BoNT-gene clusters in Clostridium botulinum strain NCTC 2916 were studied. The aim was to clarify the organization of genes within C. botulinum type A strains possessing an unexpressed BoNT/B gene. The BoNT/A-gene cluster includes genes encoding BoNT, NTNH and a part of P-47 (the gene for this protein was reported in strains of C. botulinum types E and F). Clustered with the silent BoNT/B gene were genes encoding NTNH, P-21 and HA-33. Sequencing analysis of the NTNHs revealed the presence of 471 amino acids identical in the type B and A gene clusters. This gene organization contrasts markedly with the purported organization in strain NCTC 2916 described by Henderson et al. (FEMS Microbiol. Lett. 140, 151-158). In type A(B) strain NCTC 2916, the neurotoxin gene is of type BoNT/A1 within a gene cluster that has identical organization to that found in BoNT/A2 type strains; these observations may be significant in establishing the origin of the BoNT-gene cluster
Bibliography:1997090311
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ISSN:0343-8651
1432-0991
DOI:10.1007/s002849900299