Analysis of blood and nasal epithelial transcriptomes to identify mechanisms associated with control of SARS-CoV-2 viral load in the upper respiratory tract

• Published in: The Journal of infection Vol. 87; no. 6; pp. 538 - 550
• Main Authors: Moradi Marjaneh, Mahdi, Challenger, Joseph D., Salas, Antonio, Gómez-Carballa, Alberto, Sivananthan, Abilash, Rivero-Calle, Irene, Barbeito-Castiñeiras, Gema, Foo, Cher Y., Wu, Yue, Liew, Felicity, Jackson, Heather R., Habgood-Coote, Dominic, D’Souza, Giselle, Nichols, Samuel J., Wright, Victoria J., Levin, Michael, Kaforou, Myrsini, Thwaites, Ryan S., Okell, Lucy C., Martinón-Torres, Federico, Cunnington, Aubrey J.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2023
• Subjects: Adolescent; Adult; Aged; Child; Child, Preschool; COVID-19; Female; Gene network analysis; Humans; Interferon-alpha; Male; Mathematical modelling; Middle Aged; Nasal Mucosa; Prostaglandins; SARS-CoV-2; SARS-CoV-2 - genetics; Transcriptome; Upper respiratory tract; Viral Load; Young Adult; C7orf33; Upper respiratory tract; GALNT17; IFNA2; Transcriptome; RORC; JAK/STAT; IL-22; WGCNA; IL22RA2; LRT; COVID-19; IL4I1; SARS-CoV-2; ISGs; GNLY; URT; CCL4; TUBB1; Mathematical modelling; CpG; CYP2F1; IFNL3; Viral load; Gene network analysis; NK cells; IFNA14; UGT1A1; Ct value; PTK2; PCA; IFNLR; Th22; STING1; RBP3; RNA-Seq; COL4A4; FEZ1; VL; TLR9; AIPL1; IPA; IFNAR1 and IFNAR2
• ISSN: 0163-4453; 1532-2742; 1532-2742
• DOI: 10.1016/j.jinf.2023.10.009

The amount of SARS-CoV-2 detected in the upper respiratory tract (URT viral load) is a key driver of transmission of infection. Current evidence suggests that mechanisms constraining URT viral load are different from those controlling lower respiratory tract viral load and disease severity. Understanding such mechanisms may help to develop treatments and vaccine strategies to reduce transmission. Combining mathematical modelling of URT viral load dynamics with transcriptome analyses we aimed to identify mechanisms controlling URT viral load. COVID-19 patients were recruited in Spain during the first wave of the pandemic. RNA sequencing of peripheral blood and targeted NanoString nCounter transcriptome analysis of nasal epithelium were performed and gene expression analysed in relation to paired URT viral load samples collected within 15 days of symptom onset. Proportions of major immune cells in blood were estimated from transcriptional data using computational differential estimation. Weighted correlation network analysis (adjusted for cell proportions) and fixed transcriptional repertoire analysis were used to identify associations with URT viral load, quantified as standard deviations (z-scores) from an expected trajectory over time. Eighty-two subjects (50% female, median age 54 years (range 3–73)) with COVID-19 were recruited. Paired URT viral load samples were available for 16 blood transcriptome samples, and 17 respiratory epithelial transcriptome samples. Natural Killer (NK) cells were the only blood cell type significantly correlated with URT viral load z-scores (r = −0.62, P = 0.010). Twenty-four blood gene expression modules were significantly correlated with URT viral load z-score, the most significant being a module of genes connected around IFNA14 (Interferon Alpha-14) expression (r = −0.60, P = 1e-10). In fixed repertoire analysis, prostanoid-related gene expression was significantly associated with higher viral load. In nasal epithelium, only GNLY (granulysin) gene expression showed significant negative correlation with viral load. Correlations between the transcriptional host response and inter-individual variations in SARS-CoV-2 URT viral load, revealed many molecular mechanisms plausibly favouring or constraining viral replication. Existing evidence corroborates many of these mechanisms, including likely roles for NK cells, granulysin, prostanoids and interferon alpha-14. Inhibition of prostanoid production and administration of interferon alpha-14 may be attractive transmission-blocking interventions. [Display omitted] •SARS-CoV-2 upper respiratory viral z-scores were correlated with host response.•Negative correlation between viral load and blood NK cells and IFN-α14 module.•Positive correlation between blood prostanoid module and viral load.•Nasal granulysin expression negatively correlated with viral load z-score.•Identifies putative mechanisms favouring and restricting SARS-CoV-2 replication.