Polymorphism distribution of RYR1, PRKAG3, HFABP, MYF-5 and MC4R genes in crossbred pigs

• Published in: Molecular biology reports Vol. 45; no. 6; pp. 1575 - 1585
• Main Authors: Saini, B. L., Gaur, G. K., Sahoo, N. R., Mendiratta, S. K., Kumar, A., Naha, B. C., Baranwal, A., Yadav, V., Jaiswal, R. K.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.12.2018; Springer Nature B.V
• Subjects: Alleles; AMP-Activated Protein Kinases - genetics; Animal Anatomy; Animal Biochemistry; Animal Husbandry - methods; Animals; Biomedical and Life Sciences; Breeding; Fatty Acid Binding Protein 3 - genetics; Food Quality; Gene Frequency - genetics; Gene polymorphism; Genetic Association Studies; Genotype; Haplotypes; Histology; Hogs; India; Life Sciences; Linkage Disequilibrium; Meat - analysis; Melanocortins - genetics; Morphology; Myogenic Regulatory Factor 5 - genetics; Original Article; Phenotype; Physical growth; Polymorphism, Genetic - genetics; Pork; Quantitative Trait Loci; Red Meat - analysis; Ryanodine Receptor Calcium Release Channel - genetics; Ryanodine Receptor Calcium Release Channel - metabolism; Ryanodine receptors; Single-nucleotide polymorphism; Sus scrofa - genetics; Swine - genetics; Weaning; India; Crossbred pig; PCR–RFLP; SNP; Pork quality
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-018-4263-z

This study was designed to screen the crossbred pigs for SNPs in five candidate genes, associated with pork quality traits and to differentiate their genotypes by PCR–RFLP. The results indicated that genotypes of crossbred pigs were NN (90%) and Nn (10%) for RYR1; RR (83%) and QR (17%) for PRKAG3; HH (98%), Hh (1%) and hh (1%) for HFABP; DD (99%) and CD (1%) for MYF-5; and AG (57%), GG (26%) and AA (17%) for MC4R SNPs, respectively. Allelic frequencies for five SNPs {RYR1 (1843C>T), PRKAG3 (c.599G>A), HFABP (c.1322C>T), MYF-5 (c.1205A>C) and MC4R (c.1426A>G)} were 0.95 and 0.05 (N/n), 0.08 and 0.92 (Q/R), 0.99 and 0.01 (H/h), 0.00 and 1.00 (C/D) and 0.45 and 0.55 (A/G), respectively. The effect of RYR1 (1843C>T) SNP was significant on pH 45 (P < 0.05), pH 24 (P < 0.05) and protein % (P < 0.05). The PRKAG3 (c.599G>A) and MC4R (c.1426A>G) SNP had significant association with dressing percentages. The results revealed that RYR1, PRKAG3 and MC4R SNPs may be used in marker associated selection for pork quality traits in crossbred pigs.