In vitro and cellular antioxidant activities of seaweed extracts prepared from five brown seaweeds harvested in spring from the west coast of Ireland

• Published in: Food chemistry Vol. 126; no. 3; pp. 1064 - 1070
• Main Authors: O’Sullivan, A.M., O’Callaghan, Y.C., O’Grady, M.N., Queguineur, B., Hanniffy, D., Troy, D.J., Kerry, J.P., O’Brien, N.M.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.06.2011; [Amsterdam]: Elsevier Science; Elsevier
• Subjects: antioxidant activity; Ascophyllum nodosum; Biological and medical sciences; Brown seaweed; CAT; catalase; cell lines; Comet assay; DNA damage; DPPH; edible seaweed; enzyme activity; Food industries; FRAP; Fucus serratus; Fucus vesiculosus; Fundamental and applied biological sciences. Psychology; glutathione; GSH; in vitro studies; in vivo studies; Laminaria; Laminaria hyperborea; Methanolic extract; Pelvetia canaliculata; Phaeophycophyta; plant extracts; protective effect; SOD; species differences; superoxide dismutase; Total Phenol Content; β-Carotene bleaching; Irish Republic; Europe; Ireland; ANE, Eire; Brown seaweed; DPPH; β-Carotene bleaching; CAT; Methanolic extract; Total Phenol Content; SOD; FRAP; GSH; Comet assay; Carotene; Algae; Harvesting; Extract; Antioxidant; In vitro; Phenol; Bleaching; Pigments; Phenols; Carotenoid
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2010.11.127

► Seaweed extracts reduced hydrogen peroxide-induced cell DNA damage. ► Seaweed extracts reduced H2O2-induced superoxide dismutase depletion. ► Seaweed extracts scavenged DPPH radicals. ► Seaweed extracts exhibited FRAP activities. ► Some seaweed extracts prevented β-carotene bleaching. The antioxidant potential of methanolic extracts of brown seaweeds was assessed by Total Phenol Content, Ferric Reducing Antioxidant Power (FRAP), β-carotene bleaching and the DPPH scavenging assays. Ascophyllum nodosum, Pelvetia canaliculata, and Fucus serratus contained the highest phenol concentrations while Fucus vesiculosus and F. serratus exhibited the highest FRAP activities. F. vesiculosus and A. nodosum were the most effective extracts at scavenging DPPH radicals and preventing β-carotene bleaching. The antioxidant activity of the seaweed extracts was also evaluated in Caco-2 cells. All extracts significantly (P<0.05) increased glutathione (GSH) content of cells after 24h. Caco-2 cells were also pre-treated with seaweed extract for 24h followed by exposure to hydrogen peroxide (H2O2). Antioxidant enzyme activity (catalase (CAT) and superoxide dismutase (SOD)) was assessed and DNA damage was measured using the comet assay. P. canaliculata was the most effective at preventing H2O2-mediated SOD depletion in Caco-2 cells while F. serratus exhibited the best DNA protective effects.