Pretreatment Prevotella-to-Bacteroides ratio and salivary amylase gene copy number as prognostic markers for dietary weight loss

• Published in: The American journal of clinical nutrition Vol. 111; no. 5; pp. 1079 - 1086
• Main Authors: Hjorth, Mads F, Christensen, Lars, Larsen, Thomas M, Roager, Henrik M, Krych, Lukasz, Kot, Witold, Nielsen, Dennis S, Ritz, Christian, Astrup, Arne
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2020; Oxford University Press; American Society for Clinical Nutrition, Inc
• Subjects: Adult; amylase; Amylases; Bacteroides - genetics; Bacteroides - growth & development; Biomarkers; Body weight; Body weight loss; Colon; Copy number; Diet; Dietary fiber; enterotypes; Feces - microbiology; Female; Gastrointestinal Microbiome; Gene Dosage; genes; Grain; health; Humans; Intestinal microflora; Male; Medical prognosis; Microbiomes; Microbiota; Middle Aged; Overweight - diet therapy; Overweight - enzymology; Overweight - genetics; Overweight - microbiology; Pretreatment; Prevotella; Prevotella - genetics; Prevotella - growth & development; Prognosis; Salivary alpha-Amylases - genetics; Starch; Sugar; Waist Circumference; weight; Weight control; Weight Loss; microbiota; ADD; genes; NND; weight; health; Prevotella; CNV; P/B ratio; CN; amylase; enterotypes; ddPCR; AMY1
• ISSN: 0002-9165; 1938-3207; 1938-3207
• DOI: 10.1093/ajcn/nqaa007

The inconsistent link observed between salivary amylase gene copy number (AMY1 CN) and weight management is likely modified by diet and microbiome. Based on analysis of a previously published study, we investigated the hypothesis that interaction between diet, Prevotella-to-Bacteriodes ratio (P/B ratio), and AMY1 CN influence weight change. Sixty-two people with increased waist circumference were randomly assigned to receive an ad libitum New Nordic Diet (NND) high in dietary fiber, whole grain, intrinsic sugars, and starch or an Average Danish (Western) Diet (ADD) for 26 weeks. All foods were provided free of charge. Before subjects were randomly assigned to receive the NND or ADD diet, blood and fecal samples were collected, from which AMY1 CN and P/B ratio, respectively, were determined. Body weight change was described by using linear mixed models, including biomarker [log10(P/B ratio) and/or AMY1 CN] diet-group interactions. Baseline means ± SDs of log10(P/B ratio) and AMY1 CN were −2.1 ± 1.8 and 6.6 ± 2.4, respectively. Baseline P/B ratio predicted a 0.99-kg/unit (95% CI: 0.40, 1.57; n = 54; P < 0.001) higher weight loss for those subjects on the NND compared with those on the ADD diet, whereas AMY1 CN was not found to predict weight loss differences between the NND and ADD groups [0.05 kg/CN (95% CI: −0.40, 0.51; n = 54; P = 0.83)]. However, among subjects with low AMY1 CN (<6.5 copies), baseline P/B ratio predicted a 2.12-kg/unit (95% CI: 1.37, 2.88; n = 30; P < 0.001) higher weight loss for the NND group than the ADD group. No such differences in weight loss were found among subjects in both groups with high AMY1 CN [−0.17 kg/unit (95% CI: −1.01, 0.66; n = 24; P = 0.68)]. The combined use of low AMY1 CN and pretreatment P/B ratio for weight loss prediction led to highly individualized weight loss results with the introduction of more fiber, whole grain, intrinsic sugars, and starch in the diet. These preliminary observations suggest that more undigested starch reaches the colon in individuals with low AMY1 CN, and that the fate of this starch depends on the gut microbiota composition. This trial was registered at clinicaltrials.gov as NCT01195610.