Enhancement of Colony-stimulating Activity Production by Lithium

Since lithium causes granulocytosis in some patients, its effect upon granulocyte production was investigated using mouse marrow in the agar culture system. When lithium was added to semisolid cultures of mouse marrow, there was no stimulation of colony formation in the absence of colony-stimulating...

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Bibliographic Details
Published inBlood Vol. 49; no. 2; pp. 263 - 267
Main Authors Harker, W.G., Rothstein, G., Clarkson, D., Athens, J.W., Macfarlane, J.L.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.02.1977
Subjects
Animals
Bone Marrow Cells
Cell Differentiation
Colony-Stimulating Factors - antagonists & inhibitors
Colony-Stimulating Factors - biosynthesis
Dose-Response Relationship, Drug
Glycoproteins - biosynthesis
Granulocytes - cytology
Lithium - pharmacology
Male
Mice
Mice, Inbred CBA
Puromycin - pharmacology
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Summary:Since lithium causes granulocytosis in some patients, its effect upon granulocyte production was investigated using mouse marrow in the agar culture system. When lithium was added to semisolid cultures of mouse marrow, there was no stimulation of colony formation in the absence of colony-stimulating activity (CSA). In addition, lithium did not potentiate the action of already formed CSA. However, lithium did stimulate the production of CSA by lung tissue. Lithium enhancement of CSA production was blocked by puromycin, indicating that lithium action required active new protein synthesis. It was concluded that lithium promoted enhanced granulocyte production in vitro by stimulating the synthesis of CSA.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V49.2.263.263