Entamoeba invadens: Identification of a SERCA protein and effect of SERCA inhibitors on encystation

• Published in: Microbial pathogenesis Vol. 89; pp. 18 - 26
• Main Authors: Martinez-Higuera, Aaron, Herrera-Martinez, Mayra, Chavez-Munguia, Bibiana, Valle-Solis, Martha, Muniz-Lino, Marcos A, Cazares-Apatiga, Javier, Rodriguez, Mario A
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2015
• Subjects: Calcium homeostasis; Calcium-Transporting ATPases - analysis; Calcium-Transporting ATPases - antagonists & inhibitors; Calcium-Transporting ATPases - genetics; Calreticulin - analysis; Cyclopiazonic acid; Encystation; Entamoeba - drug effects; Entamoeba - growth & development; Entamoeba histolytica; Entamoeba invadens; Enzyme Inhibitors - metabolism; Indoles - metabolism; Microscopy, Confocal; Microscopy, Fluorescence; Protozoan Proteins - analysis; Protozoan Proteins - antagonists & inhibitors; Protozoan Proteins - genetics; SERCA; Spores, Protozoan - drug effects; Spores, Protozoan - growth & development; Thapsigargin; Thapsigargin - metabolism; Transport Vesicles - chemistry; Thapsigargin; Entamoeba invadens; SERCA; Cyclopiazonic acid; Encystation; Calcium homeostasis
• ISSN: 0882-4010; 1096-1208
• DOI: 10.1016/j.micpath.2015.08.016

Calcium has an important role on signaling of different cellular processes, including growth and differentiation. Signaling by calcium also has an essential function in pathogenesis and differentiation of the protozoan parasites Entamoeba histolytica and Entamoeba invadens. However, the proteins of these parasites that regulate the cytoplasmic concentration of this ion are poorly studied. In eukaryotic cells, the calcium-ATPase of the SERCA type plays an important role in calcium homeostasis by catalyzing the active efflux of calcium from cytoplasm to endoplasmic reticulum. Here, we reported the identification of SERCA of E. invadens (EiSERCA). This protein contains a putative sequence for endoplasmic reticulum retention and all domains involved in calcium transport identified in mammalian SERCA. By immunofluorescence assays, an antibody against SERCA of E. histolytica detected EiSERCA in a vesicular network in the cytoplasm of E. invadens trophozoites, co-localizing with calreticulin. Interestingly, EiSERCA was redistributed close to plasma membrane during encystation, suggesting that this pump could participate in regulate the calcium concentration during this process. In addition, thapsigargin and cyclopiazonic acid, both specific inhibitors of SERCA, affected the number and structure of cysts, supporting the hypothesis that calcium flux mediated by SERCA has an important role in the life cycle of Entamoeba. [Display omitted] •Entamoeba invadens has a calcium pump of the SERCA type (EiSERCA).•In trophozoites EiSERCA co-localizes with calreticulin in a vesicular network distributed in all the cytoplasm.•In cyst, vesicles containing EiSERCA are translocated close to plasma membrane.•Thapsigargin and Cyclopiazonic acid, specific inhibitors of SERCA, reduced the number of cysts and altered their structure.