Comparison of two methods of high-molecular-weight DNA isolation from human leucocytes

Among the different methods for isolation of high-molecular-weight DNA from leucocytes, two of them were retained as being the most interesting and were compared. This choice was based on three criteria: the quality of the DNA obtained (high-molecular-weight DNA, RNA, and protein free), the efficien...

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Published inAnalytical biochemistry Vol. 134; no. 2; pp. 320 - 324
Main Authors Gautreau, Chantal, Rahuel, Cécile, Cartron, Jean-Pierre, Lucotte, Gérard
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 15.10.1983
Elsevier
Subjects
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Blood Proteins - analysis
Chemical Phenomena
Chemistry
DNA - blood
Dna, deoxyribonucleoproteins
Fundamental and applied biological sciences. Psychology
Humans
Leukocytes - analysis
Molecular Weight
Nucleic acids
RNA - blood
Nucleic acid
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Summary:Among the different methods for isolation of high-molecular-weight DNA from leucocytes, two of them were retained as being the most interesting and were compared. This choice was based on three criteria: the quality of the DNA obtained (high-molecular-weight DNA, RNA, and protein free), the efficiency of the method (in terms of the yield of DNA obtained), and the ease and length of the method. The first method described was chosen as a reference (100% efficiency); in comparison, the second one had a 70% efficiency, but was shorter and easier to handle.
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ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(83)90304-4