An integrated microcatheter-based dual-analyte sensor system for simultaneous, real-time measurement of propofol and fentanyl

• Published in: Talanta (Oxford) Vol. 218; p. 121205
• Main Authors: Moonla, Chochanon, Goud, K. Yugender, Teymourian, Hazhir, Tangkuaram, Tanin, Ingrande, Jerry, Suresh, Preetham, Wang, Joseph
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2020
• Subjects: Anesthesia monitoring; Anesthetic drugs; blood sampling; catheters; chemical analysis; coatings; Continuous monitoring; Electrochemical Techniques; electrochemistry; Electrodes; electroplating; Fentanyl; Gold; graphene oxide; Metal Nanoparticles; Microcatheter sensor; monitoring; nanogold; pharmacokinetics; poly(vinyl chloride); polytetrafluoroethylene; Propofol; sensors (equipment); silver; silver chloride; Simultaneous detection; surgery; uncertainty; voltammetry; Microcatheter sensor; Continuous monitoring; Simultaneous detection; Anesthesia monitoring; Fentanyl; Propofol; Anesthetic drugs
• ISSN: 0039-9140; 1873-3573; 1873-3573
• DOI: 10.1016/j.talanta.2020.121205

According to the American Society of Anesthesiologists Closed Claims Database, one of three drug-related errors is the result administrating an incorrect dose. Directly measuring drug concentration removes the uncertainty in the dose-concentration relationship and addresses inter- and intra-subject variabilities that affect the pharmacokinetics of anesthetics. Here we describe a dual-analyte microcatheter-based electrochemical sensor capable of simultaneous real-time continuous monitoring of fentanyl (FTN) and propofol (PPF) drugs simultaneously in the operating rooms. Such a dual PPF/FTN catheter sensor relies on embedding two different modified carbon paste (CP)-packed working electrodes along with Ag/AgCl microwire reference electrodes within a mm-wide Teflon tube, and uses a square wave voltammetric (SWV) technique. The composition of each working electrode was judiciously tailored to cover the concentration range of interest for each analyte. A polyvinyl chloride (PVC) organic polymer coating on the surface of CP electrode enabled selective and sensitive PPF measurements in μM range. The detection of nM FTN levels was achieved through a multilayered nanostructure-based surface modification protocol, including a CNT-incorporated CP transducer modified by a hybrid of electrodeposited Au nanoparticles and electrochemically reduced graphene oxide (erGO) and a PVC outer membrane. The long-term monitoring capability of the dual sensor was demonstrated in a protein-rich artificial plasma medium. The promising antibiofouling behavior of the catheter-based multiplexed sensor was also illustrated in whole blood samples. The new integrated dual-sensor microcatheter platform holds considerable promise towards real-time, in-vivo detection of the anesthetic drugs, propofol and fentanyl, during surgical procedures towards significantly improved safe delivery of anesthetic drugs. [Display omitted] •Integrated microcatheter-based dual-sensing probe for monitoring of anesthetic drugs.•Real-time, continuous simultaneous in-vivo monitoring of Fentanyl and Propofol in whole blood samples.•Detection of drugs in largely different concentrations without cross talk.•Continuous drugs monitoring during surgical operations towards further medical safety control.