Development of a recombinant antibody ELISA test for the detection of Polymyxa betae and its use in resistance screening

• Published in: Plant pathology Vol. 52; no. 6; pp. 673 - 680
• Main Authors: Kingsnorth, C. S., Asher, M. J. C., Keane, G. J. P., Chwarszczynska, D. M., Luterbacher, M. C., . Mutasa‐Göttgens, E. S
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.12.2003; Blackwell; Wiley Subscription Services, Inc
• Subjects: Beet necrotic yellow vein virus; Beta vulgaris; Biological and medical sciences; ELISA; Fundamental and applied biological sciences. Psychology; glutathione‐S‐transferase; His.‐tag fusion protein; Phytopathology. Animal pests. Plant and forest protection; Polymyxa betae; rhizomania; sugarbeet; Protozoa; Plant pathology; glutathione-S-transferase; Enzyme; Transferases; rhizomania; Polymyxa betae; His.-tag fusion protein; sugarbeet; Beta vulgaris var. saccharata; Sugar plant; Chenopodiaceae; Glutathione transferase; Dicotyledones; Angiospermae; Spermatophyta; Fusion protein; ELISA assay; Mycetozoa; ELISA
• ISSN: 0032-0862; 1365-3059
• DOI: 10.1111/j.1365-3059.2003.00928.x

An ELISA test was developed for the quantitative detection of the obligate parasite Polymyxa betae, the vector of Beet necrotic yellow vein virus (BNYVV), in infected sugarbeet roots. The test used monoclonal and polyclonal antibodies raised to a recombinantly expressed glutathione‐S‐transferase (GST) from P. betae. A close correlation was found between the number of P. betae zoospores in serially diluted suspensions and absorbance values in the ELISA test. Time‐course studies of plants grown in naturally infested soils in controlled environment tests demonstrated the value of the ELISA test in screening for P. betae resistance. In preliminary tests, P. betae‐resistant accessions of the wild sea beet (Beta vulgaris ssp. maritima), which might be used to restrict the transmission of BNYVV, were identified.