Ultrastructural Effects of Lactoferrin Binding on Giardia lamblia Trophozoites
Lactoferrin and its derived N‐terminal peptide may be important host defenses against Giardia lamblia. We showed earlier that lactoferrin and the derived peptides have potent giardicidal activity in vitro. Using indirect immunofluorescence, we now demonstrate binding of lactoferrin and the peptides...
Saved in:
Published in | The Journal of eukaryotic microbiology Vol. 44; no. 1; pp. 68 - 72 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.01.1997
Blackwell |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Lactoferrin and its derived N‐terminal peptide may be important host defenses against Giardia lamblia. We showed earlier that lactoferrin and the derived peptides have potent giardicidal activity in vitro. Using indirect immunofluorescence, we now demonstrate binding of lactoferrin and the peptides to the live trophozoite surface. Iron strongly inhibited binding of lactoferrin, and decreased binding of the peptides, while certain divalent metal ions decreased binding of all forms by about half. Lactoferrin and the peptedes caused striking and complex morphologic changes in the trophozoite plasmalemma, endomembranes and cytoskeleton, and increases the electron density of the lysosome‐like peripheral vacuoles. |
---|---|
Bibliography: | ark:/67375/WNG-JH9H6JMR-G istex:B38EB21C9AB4585B779CB1D645B870F0FF916FA8 ArticleID:JEU68 Current address: Rock Valley College, 3301 North Mulford Road, Rockford, Illinois 61 I14. Current address: Department of Biological Sciences, University oj Texas, El Paso, El Paso Texas 79968‐0590. ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 1066-5234 1550-7408 |
DOI: | 10.1111/j.1550-7408.1997.tb05694.x |