Mutations that alter the helix-turn-helix region of the spollAC protein: a Bacillus subtilis sporulation-specific sigma factor

• Published in: Molecular microbiology Vol. 3; no. 2; p. 257
• Main Authors: Yudkin, M D, Millonig, J H, Appleby, L
• Format: Journal Article
• Language: English
• Published: England 01.02.1989
• Subjects: Amino Acids - analysis; Bacillus subtilis - genetics; Bacillus subtilis - physiology; Bacterial Proteins - genetics; Cloning, Molecular; Codon; DNA Mutational Analysis; DNA, Bacterial; Protein Conformation; Restriction Mapping; Sigma Factor - genetics; Spores, Bacterial - growth & development; Suppression, Genetic; Transcription Factors - genetics
• DOI: 10.1111/j.1365-2958.1989.tb01815.x
• ISSN: 0950-382X

It has previously been shown that spollAC561, a mutation that diminishes the incidence of sporulation by more than six orders of magnitude, alters the residue at position 13 of the helix-turn-helix region of the sporulation-specific sigma factor encoded by spollAC from valine to methionine (Yudkin, 1987b). We have now found that four spontaneous revertants, which sporulate at an incidence of 30-60%, all contain transitions within the codon that was altered by spo-561. The mutant methionine is replaced by isoleucine in two revertants, and by threonine in the other two.