Mutations that alter the helix-turn-helix region of the spollAC protein: a Bacillus subtilis sporulation-specific sigma factor

It has previously been shown that spollAC561, a mutation that diminishes the incidence of sporulation by more than six orders of magnitude, alters the residue at position 13 of the helix-turn-helix region of the sporulation-specific sigma factor encoded by spollAC from valine to methionine (Yudkin,...

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Bibliographic Details
Published inMolecular microbiology Vol. 3; no. 2; p. 257
Main Authors Yudkin, M D, Millonig, J H, Appleby, L
Format Journal Article
LanguageEnglish
Published England 01.02.1989
Subjects
Amino Acids - analysis
Bacillus subtilis - genetics
Bacillus subtilis - physiology
Bacterial Proteins - genetics
Cloning, Molecular
Codon
DNA Mutational Analysis
DNA, Bacterial
Protein Conformation
Restriction Mapping
Sigma Factor - genetics
Spores, Bacterial - growth & development
Suppression, Genetic
Transcription Factors - genetics
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Summary:It has previously been shown that spollAC561, a mutation that diminishes the incidence of sporulation by more than six orders of magnitude, alters the residue at position 13 of the helix-turn-helix region of the sporulation-specific sigma factor encoded by spollAC from valine to methionine (Yudkin, 1987b). We have now found that four spontaneous revertants, which sporulate at an incidence of 30-60%, all contain transitions within the codon that was altered by spo-561. The mutant methionine is replaced by isoleucine in two revertants, and by threonine in the other two.
ISSN:0950-382X
DOI:10.1111/j.1365-2958.1989.tb01815.x