Effects of Raloxifene on Caveolin‐1 mRNA and Protein Expressions in Vascular Smooth Muscle Cells

• Published in: Acta biochimica et biophysica Sinica Vol. 38; no. 11; pp. 747 - 752
• Main Authors: YANG, Fa‐Lin, HE, Hong, LIU, Xian‐Xi, TU, Bing, ZENG, Xian‐Wei, SU, Ji‐Xin, WANG, Xin, HU, Qin
• Format: Journal Article
• Language: English
• Published: Melbourne, Australia Blackwell Publishing Asia 01.11.2006
• Subjects: Animals; Aorta - cytology; Cardiotonic Agents - pharmacology; Caveolin 1 - biosynthesis; caveolin‐1; Cells, Cultured; Estradiol - analogs & derivatives; Estradiol - pharmacology; estrogen receptor; Female; Fulvestrant; Gene Expression Regulation; Myocytes, Smooth Muscle - drug effects; Myocytes, Smooth Muscle - metabolism; raloxifene; Raloxifene Hydrochloride - pharmacology; Rats; Rats, Wistar; Receptors, Estrogen - agonists; Receptors, Estrogen - antagonists & inhibitors; Receptors, Estrogen - physiology; RNA, Messenger - biosynthesis; Selective Estrogen Receptor Modulators - pharmacology; vascular smooth muscle cell
• ISSN: 1672-9145; 1745-7270
• DOI: 10.1111/j.1745-7270.2006.00222.x

Caveolin‐1 is regulated by estrogen in vascular smooth muscle cells. Raloxifene, a selective estrogen receptor modulator that possibly has cardioprotective properties without an increased risk of cancer or other side effects of estrogen, may be used in women with risk of coronary artery disease. However, the relationship between raloxifene and caveolin‐1 is still unknown. Therefore, this study was designed to see whether raloxifene regulates caveolin‐1 expression and if so, whether such regulation is mediated by estrogen receptor. Rat aortic smooth muscle cells were cultured in the absence or presence of raloxifene (10−8 to 10−6 M) for 12 or 24 h. Both mRNA and protein levels of caveolin‐1 were increased significantly after 24 h treatment with raloxifene. These increases were inhibited by estrogen receptor antagonist ICI 182780 (10−5 M). Results of this study suggest that raloxifene stimulates caveolin‐1 transcription and translation through estrogen receptor mediated mechanisms. Edited by Autonio CANO