Application of a fluorescent redox dye for enumeration of metabolically active bacteria on albumin-coated titanium surfaces

A bacterial staining method using fluorescent redox dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) is described for quantifying actively respiring bacteria that adhere to commercially pure titanium surfaces coated with cross-linked albumin. This has not been possible to date using ordinary DNA s...

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Bibliographic Details
Published inLetters in applied microbiology Vol. 21; no. 1; p. 1
Main Authors McDowell, S G, An, Y H, Draughn, R A, Friedman, R J
Format Journal Article
LanguageEnglish
Published England 01.07.1995
Subjects
Bacterial Adhesion
Bisbenzimidazole - chemistry
Colony Count, Microbial - methods
Fluorescent Dyes - metabolism
Microscopy, Fluorescence
Oxygen Consumption
Propidium - chemistry
Serum Albumin, Bovine - chemistry
Staining and Labeling
Staphylococcus - isolation & purification
Staphylococcus - metabolism
Tetrazolium Salts - metabolism
Titanium - chemistry
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Summary:A bacterial staining method using fluorescent redox dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) is described for quantifying actively respiring bacteria that adhere to commercially pure titanium surfaces coated with cross-linked albumin. This has not been possible to date using ordinary DNA stains such as propidium iodide (PI) or Hoechst, both of which produce a very bright background. With this technique, it was demonstrated that the cross-linked albumin inhibited the adherence of Staphylococcus aureus and Staph. epidermidis to the titanium surface.
ISSN:0266-8254
DOI:10.1111/j.1472-765X.1995.tb00992.x