Voltammetric Studies on the Recognition of a Copper Complex to Single- and Double-Stranded DNA and Its Application in Gene Biosensor
A mixed‐ligands copper complex [Cu(phendione)(DAP)]SO4 (phendione=1,10‐phenanthroline‐5,6‐dione, DAP=2,3‐diaminophenazine) was synthesized. Cyclic voltammetry showed that the complex underwent an obvious decrease of redox peak currents and positive shift of formal potential after interaction with do...
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Published in | Electroanalysis (New York, N.Y.) Vol. 20; no. 19; pp. 2096 - 2101 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag
01.10.2008
WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
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Summary: | A mixed‐ligands copper complex [Cu(phendione)(DAP)]SO4 (phendione=1,10‐phenanthroline‐5,6‐dione, DAP=2,3‐diaminophenazine) was synthesized. Cyclic voltammetry showed that the complex underwent an obvious decrease of redox peak currents and positive shift of formal potential after interaction with double‐stranded DNA (dsDNA), suggesting that the copper complex behaved as a typical metallointercalator for dsDNA, The recognition properties of the copper complex to single‐stranded DNA (ssDNA) and dsDNA were assessed using surface‐based electrochemical methods and the results suggested that the complex had obviously different redox signals at ssDNA and dsDNA modified electrodes. The copper complex was further used as an electroactive indicator for the detection of cauliflower mosaic virus (CaMV) 35S promoter gene. |
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Bibliography: | ArticleID:ELAN200804291 ark:/67375/WNG-Q6RJZ7S5-G istex:C222C61580761107E8A95022B47586ED770F9BB4 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 1040-0397 1521-4109 |
DOI: | 10.1002/elan.200804291 |