Insulin-like growth factor binding protein-1-6 expression in activated microglia

• Published in: Neuroreport Vol. 15; no. 6; p. 1033
• Main Authors: Chesik, Daniel, Glazenburg, Koen, Wilczak, Nadine, Geeraedts, Felix, De Keyser, Jacques
• Format: Journal Article
• Language: English
• Published: England 29.04.2004
• Subjects: Animals; Cells, Cultured; Gene Expression Regulation - drug effects; Gene Expression Regulation - physiology; Insulin-Like Growth Factor Binding Protein 1 - biosynthesis; Insulin-Like Growth Factor Binding Protein 1 - genetics; Insulin-Like Growth Factor Binding Protein 2 - biosynthesis; Insulin-Like Growth Factor Binding Protein 2 - genetics; Insulin-Like Growth Factor Binding Protein 3 - biosynthesis; Insulin-Like Growth Factor Binding Protein 3 - genetics; Insulin-Like Growth Factor Binding Protein 4 - biosynthesis; Insulin-Like Growth Factor Binding Protein 4 - genetics; Insulin-Like Growth Factor Binding Protein 5 - biosynthesis; Insulin-Like Growth Factor Binding Protein 5 - genetics; Insulin-Like Growth Factor Binding Protein 6 - biosynthesis; Insulin-Like Growth Factor Binding Protein 6 - genetics; Insulin-Like Growth Factor Binding Proteins - biosynthesis; Insulin-Like Growth Factor Binding Proteins - genetics; Lipopolysaccharides - pharmacology; Microglia - drug effects; Microglia - metabolism; Rats; Rats, Wistar; RNA, Messenger - biosynthesis; RNA, Messenger - genetics
• ISSN: 0959-4965
• DOI: 10.1097/00001756-200404290-00020

In the CNS insulin-like growth factor-1 (IGF-1) enhances survival of neurons, promotes myelin synthesis and acts as a mitogen for microglia. The effects of IGF-1 are regulated by a family of 6 IGF binding proteins (IGFBPs). We investigated mRNA expression patterns of IGFBPs in primary rat microglia under basal conditions and after activation with lipopolysaccharide (LPS). Under basal conditions, microglia expressed IGFBP-2 to -6, whereas, IGFBP-1 could not be detected. Following 2 h treatment with LPS mRNA levels for IGFBP-4 and -6 displayed a down regulation, and IGFBP-5 became undetectable. Levels of IGFBP-2 and -3 remained unaltered. Expression patterns of IGFBPs might play an important role in regulating the autocrine/paracrine IGF-1 actions on microglia under inflammatory conditions.