Biogenic Amines in Cultured Neuroblastoma and Astrocytoma Cells

The presence of biogenic amines in cultured cells of mouse neuroblastoma C-1300 (clone NB-2a) was suggested by fluorescence-microscope histochemistry. Incubation in media containing L-[14C]tyrosine and L-[14C]tryptophan for 24 h, followed by high-voltage electrophoresis, radiochromatogram scanning,...

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Published inThe Journal of cell biology Vol. 63; no. 1; pp. 64 - 70
Main Authors Narotzky, Robert, Bondareff, William
Format Journal Article
LanguageEnglish
Published United States Rockefeller University Press 01.10.1974
The Rockefeller University Press
Subjects
Amines
Animals
Astrocytoma
Astrocytoma - analysis
Biogenic Amines - analysis
Carbon Radioisotopes
Catecholamines
Cell Line
Cell lines
Cells
Cells, Cultured
Clone Cells
Cultured cells
Dopamine - analysis
Electrophoresis, Paper
Epinephrine - analysis
Fluorescence
Formaldehyde
Histocytochemistry
Mice
Microscopy, Fluorescence
Neoplasms, Nerve Tissue
Neuroblastoma
Neuroblastoma - analysis
Neurons
Norepinephrine - analysis
Octopamine - analysis
Rats
Serotonin - analysis
Spectrometry, Fluorescence
Stem cells
Stereoisomerism
Tryptophan
Tyramine - analysis
Tyrosine
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Summary:The presence of biogenic amines in cultured cells of mouse neuroblastoma C-1300 (clone NB-2a) was suggested by fluorescence-microscope histochemistry. Incubation in media containing L-[14C]tyrosine and L-[14C]tryptophan for 24 h, followed by high-voltage electrophoresis, radiochromatogram scanning, and scintillation counting, confirmed the presence of [14C]dopamine, [14C]norepinephrine, [14C]epinephrine, [14C]serotonin, [14C]tyramine, and [14C]octopamine. Dopamine, norepinephrine, epinephrine, and serotonin were demonstrated spectrophoto-fluorometrically in concentrations, expressed as micrograms amine per milligram protein, of 1.19, 0.027, 0.038, and 0.148, respectively, for cells in a stationary growth phase. Fluorescence-microscope histochemistry also suggested the presence of biogenic amines in cultured astrocytoma cells (cell line C6). Spectrophotofluorometric assay of cells in a stationary growth phase demonstrated intracellular dopamine, norepinephrine, epinephrine, and serotonin in concentrations significantly lower than those of neuroblastoma cells.
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content type line 23
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.63.1.64