Myocardial m6A regulators in postnatal development: effect of sex

N6-methyladenosine (m6A) is an abundant mRNA modification affecting mRNA stability and protein expression. It is a highly dynamic process, and its outcomes during postnatal heart development are poorly understood. Here we studied m6A machinery in the left ventricular (LV) myocardium of Fisher344 mal...

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Published inPhysiological research Vol. 71; no. 6; pp. 877 - 882
Main Authors Semenovykh, D, Benak, D, Holzerova, K, Cerna, B, Telensky, P, Vavrikova, T, Kolar, F, Neckar, J, Hlavackova, M
Format Journal Article
LanguageEnglish
Published Czech Republic Institute of Physiology 16.12.2022
Institute of Physiology of the Czech Academy of Sciences
Subjects
Animals
Epigenetics
Female
Females
Gender differences
Gene expression
Heart
Laboratory animals
Male
Males
Methyltransferase
mRNA stability
Myocardium
N6-methyladenosine
Protein expression
Proteins
Rats
RNA modification
Sexes
Short Communication
Statistical significance
Ventricle
United Kingdom > UK
United States > US
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Summary:N6-methyladenosine (m6A) is an abundant mRNA modification affecting mRNA stability and protein expression. It is a highly dynamic process, and its outcomes during postnatal heart development are poorly understood. Here we studied m6A machinery in the left ventricular (LV) myocardium of Fisher344 male and female rats (postnatal days one to ninety; P1-P90) using Western Blot. A downward pattern of target protein levels (demethylases FTO and ALKBH5, methyltransferase METTL3, reader YTHDF2) was revealed in male and female rat LVs during postnatal development. On P1, the FTO protein level was significantly higher in male LVs compared to females.
ISSN:0862-8408
1802-9973
DOI:10.33549/physiolres.934970