Unit-length, single-stranded circular DNAs of both polarity of begomoviruses are generated in Escherichia coli harboring phage M13-cloned begomovirus genome with single copy of replication origin

• Published in: Virus research Vol. 125; no. 1; pp. 14 - 28
• Main Authors: Wu, Chia-Ying, Yang, Su-Hua, Lai, Yi-Chin, Lin, Na-Sheng, Hsu, Yau-Heiu, Hu, Chung-Chi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2007
• Subjects: Ageratum yellow vein virus; Bacteriophage M13 - genetics; Begomovirus; Begomovirus - genetics; Begomoviruses; Coliphages - genetics; DNA, Circular - genetics; DNA, Circular - metabolism; DNA, Single-Stranded; DNA, Viral - genetics; DNA, Viral - metabolism; Escherichia coli; Escherichia coli - genetics; Escherichia coli - virology; Genetic Engineering - methods; Genome, Viral; Phage M13; Plasmids - genetics; Replication Origin; Replication-associated protein; Single-stranded circular DNA; Phage M13; Replication-associated protein; Single-stranded circular DNA; Escherichia coli; Begomoviruses

Replication of genomic DNAs of plant-pathogenic begomoviruses has been demonstrated in prokaryotes, which supported the possibility of analyzing DNA replication process of begomoviruses in bacteria. However, previous studies indicated that the replication of begomovirus DNAs in prokaryotes requires...