mRNA expression of novel CGRP1 receptors and their activity-modifying proteins in hypoxic rat lung
Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53706 Calcitonin gene-related peptide (CGRP) is a potent vasodilator. Our group has reported that exogenous CGRP may prevent or reverse hypoxic pulmonary hypertension in rats. Th...
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Published in | American journal of physiology. Lung cellular and molecular physiology Vol. 280; no. 3; pp. 547 - L554 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
01.03.2001
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Subjects | |
Online Access | Get full text |
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Summary: | Department of Comparative Biosciences, School of Veterinary
Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53706
Calcitonin gene-related peptide
(CGRP) is a potent vasodilator. Our group has reported that exogenous
CGRP may prevent or reverse hypoxic pulmonary hypertension in rats. The
vasodilatory action of CGRP is mediated primarily by CGRP1 receptors.
The calcitonin receptor-like receptor (CRLR) and the orphan receptor
RDC-1 have been proposed as CGRP1 receptors, and recent
evidence suggests that CRLR can function as either a CGRP1 receptor or
an adrenomedullin (ADM) receptor. Receptor activity-modifying proteins
(RAMPs) determine the ligand specificity of CRLR: coexpression of CRLR
and RAMP1 results in a CGRP1 receptor, whereas coexpression of CRLR and RAMP2 or -3 results in an ADM receptor. We used qualitative,
semiquantitative, and real-time quantitative RT-PCR to detect and
quantitate the relative expression of these agents in the lungs of rats
exposed to normoxia ( n = 3) and 1 and 2 wk of chronic
hypobaric hypoxia (barometric pressure 380 mmHg, equivalent to an
inspired O 2 level of 10%; n = 3/time
period). Our results show upregulation of RDC-1, RAMP1, and RAMP3 mRNAs
in hypoxic rat lung and no change in CRLR and RAMP2 mRNAs. These
findings support a functional role for CGRP and ADM receptors in
regulating the adult pulmonary circulation.
semiquantitative reverse transcription-polymerase chain reaction; real-time quantitative reverse transcription-polymerase chain reaction; calcitonin receptor-like receptor; RDC-1; receptor activity-modifying
protein 1; receptor activity-modifying protein 2; receptor
activity-modifying protein 3 |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1040-0605 1522-1504 |
DOI: | 10.1152/ajplung.2001.280.3.l547 |