Tetracycline-resistant bacteria and ribosomal protection protein genes in soils from selected agricultural fields and livestock farms

• Published in: Applied biological chemistry Vol. 64; no. 1; pp. 1 - 9
• Main Authors: Nogrado, Kathyleen, Unno, Tatsuya, Hur, Hor-Gil, Lee, Ji-Hoon
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.12.2021; Springer Nature B.V; 한국응용생명화학회
• Subjects: Agricultural land; Antibiotic resistance; Antibiotics; Applied Microbiology; Arable land; Bacteria; Biological Techniques; Bioorganic Chemistry; Chemistry; Chemistry and Materials Science; Copy number; Drug resistance; Erythromycin; Farms; Genes; Intensive farming; Livestock; Livestock farming; Multidrug resistance; Principal components analysis; Proteins; Small-scale farming; Soil bacteria; Soil environment; Soil resistance; Soils; Streptomycin; 농학; Agricultural soil; RPP; Antibiotics-resistance; genes
• ISSN: 2468-0834; 2468-0842
• DOI: 10.1186/s13765-021-00613-6

Antibiotic resistance in soil environment has eminently been compared and studied between agricultural and pristine soils, and the role of concentrated animal feeding operations has markedly been recognized as one of the major sources of antibiotic resistance. This study described the tetracycline resistance in small-scale farms in pursuit of presenting its possible role and contribution to the persistence of antibiotic resistance in the environment. Results of the study would render additional information on the occurrence of the ribosomal protection protein (RPP) tet genes among the isolated bacteria from the selected agricultural soils. Four tetracycline resistance and RPP genes were determined in two different agricultural soil settings. Both the culture and molecular method were used to determine and measure tetracycline resistance in soils from arable land and animal house. Results revealed a significantly higher number of culturable antibiotic-resistant bacteria in animal houses than arable lands which was suggestive of higher antibiotic resistance in areas where there was direct administration of the antibiotics. However, quantification of the gene copy numbers in the agricultural soils indicated a different result. Higher gene copy number of tet O was determined in one animal house (IAH-3), while the two other tet genes tet Q and tet W were found to be higher in arable lands. Of the total 110 bacterial isolates, tet W gene was frequently detected, while tet O gene was absent in any of the culturable bacterial isolates. Principal component analysis of occurrence and gene copy number of RPP tet genes tet O, tet Q, and tet W also revealed highest abundance of RPP tet genes in the manure and arable soils. Another important highlight of this study was the similarity of the RPP tet genes detected in the isolated bacteria from the agricultural soils to the identified RPP tet genes among pathogenic bacteria. Some of the tetracycline-resistant bacterial isolates were also multidrug resistant as it displayed resistance to tetracycline, erythromycin, and streptomycin using disk diffusion testing.