Plasmon microscopy and imaging ellipsometry of Artrobacter oxydans attached on polymer films

The attachment of Artrobacter oxydans 1388 on a newly synthesized biodegradable copolymer of poly-(hexanlactam)- co-block-poly-(δ-valerolactone) is investigated by optical, microscopic and biochemical methods. The potentials of surface plasmon microscopy and imaging ellipsometry for detecting microo...

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Published inColloids and surfaces, B, Biointerfaces Vol. 65; no. 2; pp. 276 - 280
Main Authors Marinkova, Dessislava, Bivolarska, Maria, Ahtapodov, Lyubomir, Yotova, Lyubov, Mateva, Rosa, Velinov, Tzvetan
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.09.2008
Subjects
Arthrobacter - cytology
Arthrobacter - growth & development
Artrobacter oxydans
Bacterial Adhesion
Biofilms
Imaging ellipsometry
Microscopy - methods
Microscopy, Electron, Scanning
Plasmon microscopy
Surface Plasmon Resonance
Plasmon microscopy
Imaging ellipsometry
Biofilms
Artrobacter oxydans
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Summary:The attachment of Artrobacter oxydans 1388 on a newly synthesized biodegradable copolymer of poly-(hexanlactam)- co-block-poly-(δ-valerolactone) is investigated by optical, microscopic and biochemical methods. The potentials of surface plasmon microscopy and imaging ellipsometry for detecting microorganisms when Al films are used to excite plasmons is assessed by comparing images obtained by these methods with dark field microscopy pictures. The experimental results demonstrate that in this case imaging ellipsometry and plasmon microscopy in transmission are promising methods and can be used in optical sensors for monitoring cell adhesion.
Bibliography:ObjectType-Article-1
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ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2008.04.014