The effects of ruminal acidosis on volatile fatty acid absorption and plasma activities of pancreatic enzymes in lambs

• Published in: Journal of animal science Vol. 73; no. 10; p. 3111
• Main Authors: Krehbiel, C.R. (USDA, ARS, USMARC, Clay Center, NE.), Britton, R.A, Harmon, D.L, Wester, T.J, Stock, R.A
• Format: Journal Article
• Language: English
• Published: United States 01.10.1995
• Subjects: ABSORCION; ABSORPTION; Acetates - pharmacokinetics; ACIDE GRAS VOLATIL; ACIDO GRASO VOLATIL; ACIDOSE; ACIDOSIS; Acidosis - metabolism; Acidosis - physiopathology; Acidosis - veterinary; AGNEAU; AMILASAS; AMYLASE; Amylases - blood; Animals; CORDERO; Fatty Acids, Volatile - analysis; Fatty Acids, Volatile - metabolism; Fatty Acids, Volatile - pharmacokinetics; FLUIDE DU RUMEN; FLUIDO DEL RUMEN; Glucose - adverse effects; Glucose - analysis; Hydrogen-Ion Concentration; Lipase - blood; Male; PANCREAS; PLASMA SANGUIN; PLASMA SANGUINEO; Random Allocation; RUMEN; Rumen - chemistry; Rumen - metabolism; Rumen - physiopathology; Sheep; Sheep Diseases - chemically induced; Sheep Diseases - metabolism; Sheep Diseases - physiopathology; Time Factors; TRIACILGLICEROL LIPASA; TRIACYLGLYCEROL LIPASE
• ISSN: 0021-8812; 1525-3163
• DOI: 10.2527/1995.73103111x

Twenty crossbred wethers (41.9 +/- 4.0 kg BW), each fitted with a ruminal cannula and a jugular catheter, were used in a completely randomized design to examine the effects of ruminal acidosis on plasma activities of pancreatic enzymes and fractional rates of VFA absorption. Lambs had ad libitum access to a 50% concentrate diet. Acidosis was induced by an intraruminal dose of glucose at 0, 6, 12, or 18 g/kg BW via the ruminal cannula. Ruminal fluid and plasma were collected 0, 4, 8, 12, 18, 24, 36, 48, and 72 h after dosing. Ruminal fluid pH was reduced (linear, P 0.001) with increasing ruminal glucose. Total ruminal VFA concentration decreased (linear, P 0.01) and D(-)-lactate (linear, P 0.01) and L(+)-lactate (linear, P 0.10). Ten days after the acidosis insult, rumens were evacuated and contents were replaced with an isotonic Cr:VFA solution to measure ruminal VFA absorption. Ruminal fluid was collected hourly from 0 to 6 h. Fractional rate of acetate absorption was 13% lower for lambs receiving 18 g/kg BW glucose than for control lambs. In addition, fractional liquid passage rate was lower (P 0.05) in lambs receiving 18 g/kg BW glucose 6 mo after the insult of acidosis. These data suggest that a short-term, severe insult of acute acidosis does not result in pancreatic tissue damage but may result in reduced ruminal VFA absorption for an extended period of time