Electroformation in a flow chamber with solution exchange as a means of preparation of flaccid giant vesicles

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 64; no. 1; pp. 77 - 87
• Main Authors: Peterlin, Primož, Arrigler, Vesna
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.06.2008
• Subjects: Conductive solution; Electric Conductivity; Electrochemistry - instrumentation; Electrochemistry - methods; Electroformation; Giant vesicle; Glycerol; Membrane permeability; Permeability; Pharmaceutical Solutions; Phosphatidylcholines - chemistry; Phospholipids - chemical synthesis; Phospholipids - chemistry; Unilamellar Liposomes - chemical synthesis; Unilamellar Liposomes - chemistry; Glycerol; Electroformation; Giant vesicle; Conductive solution; Membrane permeability
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2008.01.004

A recently described technique [Estes and Mayer, Biochim. Biophys. Acta 1712 (2005) 152–160] for the preparation of giant unilamellar vesicles (GUVs) in solutions with high ionic strength is examined. By observing a series of osmotic swellings followed by vesicle bursts upon a micropipette transfer of a single POPC GUV from a sucrose solution into an iso-osmolar glycerol solution, a value for the permeability of POPC membrane for glycerol, P = ( 2.09 ± 0.82 ) × 1 0 − 8 m/s, has been obtained. Based on this result, an alternative mechanism is proposed for the observed exchange of vesicle interior. With modifications, the method of Estes and Mayer is then applied to preparation of flaccid GUVs.