Development and Validation of a Liquid Chromatographic Method for Monitoring of Process-Related Synthetic Organic Impurities of Profenofos in Technical Products

• Published in: Journal of chromatographic science Vol. 41; no. 8; pp. 418 - 421
• Main Authors: Rao, R. Nageswara, Raju, D. Naga, Venkateswarlu, N., Rao, B. Vittal, Parvathi, N., Manjula, A., Reddy, G.N., Gawali, P.B., Sreekanth, M., Rao, P. Nageswara
• Format: Journal Article
• Language: English
• Published: Niles, IL Oxford University Press 01.09.2003; Preston Publications
• Subjects: Biological and medical sciences; Chemical control; Chromatography, High Pressure Liquid - methods; Control; Fundamental and applied biological sciences. Psychology; Insecticides - chemistry; Organic Chemicals - analysis; Organothiophosphates - chemistry; Phytopathology. Animal pests. Plant and forest protection; Protozoa. Invertebrates; Reproducibility of Results; Sensitivity and Specificity; Spectrophotometry, Ultraviolet; Trace analysis; Validation; Elution; Gradient; Chemical analysis; Insecticide; Impurity; Pesticides; HPLC chromatography; Purity control; Reversed phase chromatography; Ultraviolet detector; Quality control; Organophosphorus compounds; Quantitative analysis
• ISSN: 0021-9665; 1945-239X
• DOI: 10.1093/chromsci/41.8.418

A simple and rapid reversed-phase high-performance liquid chromatographic method for the monitoring of process-related synthetic organic impurities of profenofos (PFS) is developed. Impurities are separated and determined on a reversed-phase Hypersil C18 column using gradient elution of 50mM ammonium formate buffer-acetonitrile as a mobile phase and detection at 230 nm at ambient temperature. The method is validated with respect to accuracy, precision, linearity, and limits of detection and quantitation. The method is found to be suitable not only for monitoring the reactions involved in the process development of PFS, but also quality assurance, as it can detect impurities at the level of 1.5 × 10−8 g.