Preliminary investigation to determine the cytotoxicity of various cryoprotectants on southern African abalone ( Haliotis midae) embryos

Cryopreservation could provide stock quantities of embryos for transgenic research. This study aimed to determine the least toxic cryoprotective agent for Haliotis midae embryos. They were exposed for 30 min to concentrations varying from 5% to 20% of the following cryopreservatives: methanol (MET),...

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Bibliographic Details
Published inCryobiology Vol. 57; no. 3; pp. 308 - 311
Main Authors Roux, A., Sandenbergh, L., Roodt-Wilding, R.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Inc 01.12.2008
Subjects
Abalone embryos
Africa, Southern
Animals
Cryoprotectants
Cryoprotective Agents - toxicity
Dimethyl Sulfoxide - toxicity
Embryo, Nonmammalian - drug effects
Embryo, Nonmammalian - embryology
Gastropoda - embryology
Glycerol - toxicity
Haliotis
Haliotis midae
Polyethylene Glycols - toxicity
Toxic effects
Africa, Southern
Toxic effects
Abalone embryos
Cryoprotectants
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Summary:Cryopreservation could provide stock quantities of embryos for transgenic research. This study aimed to determine the least toxic cryoprotective agent for Haliotis midae embryos. They were exposed for 30 min to concentrations varying from 5% to 20% of the following cryopreservatives: methanol (MET), polyethylene glycol (PEG), dimethyl sulfoxide (ME 2SO) and glycerol (GLY). In contrast to cryopreservation studies done in other molluscs, PEG showed the least toxicity to H. midae embryos in concentrations ranging from 5% to 15%. MET was also less toxic than ME 2SO and GLY at correlating concentrations. GLY showed the most toxic effects with most embryos dead or abnormal at concentrations above 15%.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2008.09.009