Treatment of corn bran dietary fiber with xylanase increases its ability to bind bile salts, in vitro

• Published in: Food chemistry Vol. 106; no. 1; pp. 113 - 121
• Main Authors: Hu, Ye-Bi, Wang, Zhang, Xu, Shi-Ying
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 2008; Elsevier
• Subjects: Bile salt-binding; bile salts; binding capacity; Biological and medical sciences; Cereal and baking product industries; corn bran; Corn bran dietary fiber; Correlation; dietary fiber; endo-1,4-beta-xylanase; enzymatic hydrolysis; enzymatic treatment; food analysis; food composition; Food industries; Fundamental and applied biological sciences. Psychology; In vitro; Increase; Response surface methodology; sodium chenodeoxycholate; sodium cholate; sodium deoxycholate; sodium taurocholate; Xylanase hydrolysis; xylanase modified fiber; Correlation; Bile salt-binding; Xylanase hydrolysis; Increase; Response surface methodology; In vitro; Corn bran dietary fiber; Ability; Binding; Cereal bran; Methodology; Enzyme; Bile salt; Corn; Hydrolysis; Glycosidases; Cereal product; Cereal; Hydrolases; Response surface; Xylan endo-1,3-β-xylosidase; Dietary fiber; O-Glycosidases
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2007.05.054

A corn bran fiber (CDF) was further treated by xylanase and the product – XMF was obtained. Response surface methodology (RSM) was used to optimize the hydrolysis conditions (pH, time and enzyme dosage), binding of cholate (B SC), chenodeoxycholate (B SCDC), deoxycholate (B SDC) and taurocholate (B STC) by XMF were determined. The influence trends of 3 factors were dissimilar, pH affected the binding capacity most significantly, then hydrolysis time, lastly the dosage. The optimized conditions were pH 5.3, 1.75 h and enzyme dosage 0.70 g/100 g CDF, the values for B SC, B SCDC, B SDC and B STC were increased to 1.88, 2.34, 1.67 and 2.08 fold of CDF, respectively, which were not significantly different from those predicted ( p < 0.05). There was not correlation between the bindings of any two bile salts by XMF, which indicates that the binding mechanisms of different bile salts by XMF studied here are different. The TDF, IDF and SDF content of XMF were increased by 12%, 12% and 285%, respectively. The WHC, SW and OBC of XMF were 1.11, 1.34 and 1.87 fold of CDF, respectively.