Sensitivity and specificity of the agar-gel- immunodiffusion test, ELISA and the skin test for detection of paratuberculosis in United States Midwest sheep populations

• Published in: Veterinary research (Paris) Vol. 37; no. 4; pp. 553 - 564
• Main Authors: ROBBE-AUSTERMAN, Suelee, GARDNER, Ian A, THOMSEN, Bruce V, MORRICAL, Daniel G, MARTIN, Barbara M, PALMER, Mitchell V, THOEN, Charles O, EWING, Chad
• Format: Journal Article
• Language: English
• Published: Les Ulis EDP Sciences 01.07.2006; BioMed Central
• Subjects: Animal biology; Animal genetics; Animals; Biochemistry, Molecular Biology; Biological and medical sciences; Cell Behavior; Cellular Biology; Enzyme-Linked Immunosorbent Assay - veterinary; Fundamental and applied biological sciences. Psychology; Genetics; Immunodiffusion - veterinary; Immunology; Life Sciences; Microbiology; Microbiology and Parasitology; Midwestern United States - epidemiology; Molecular biology; Neurons and Cognition; Paratuberculosis - diagnosis; Paratuberculosis - epidemiology; Santé publique et épidémiologie; Sensitivity and Specificity; Sheep; Sheep Diseases - diagnosis; Sheep Diseases - epidemiology; Skin Tests - veterinary; United States; North America; America; Midwestern United States; Farming animal; Microbiology; Paratuberculosis; Mycobacterial infection; Infection; Vertebrata; Sensitivity; Specificity; Mammalia; ep / ELISA / AGID / skin test; Johne's disease; Bacteriosis; Sheep; Artiodactyla; Detection; Veterinary; ELISA assay; Ungulata; Skin test; skin test; sheep; ELISA; AGID
• ISSN: 0928-4249; 1297-9716
• DOI: 10.1051/vetres:2006018

Our objective was to estimate the sensitivity and specificity of the agar-gel-immunodiffusion test (AGID), the ELISA, and the skin test for the detection of Mycobacterium avium subspecies paratuberculosis (MAP) in sheep using Bayesian methods without a gold standard. Fourteen flocks (2 465 sheep) were used. Five flocks (450 sheep) were considered MAP non-infected and 9 flocks (2 015 sheep) had sheep infected with MAP. Sheep were skin tested and blood was collected for AGID and ELISA testing. Results were analyzed using a Bayesian 3-test in 1-population model fitted in WinBUGS. The model allowed for dependence (correlation) between the two serologic tests, but these two tests were assumed to be conditionally independent of the skin test. The estimated specificity was 99.5% (95% PI of 98.9-99.9%) for the AGID; 99.3% (98.4-99.8%) for the ELISA using an optical density measured cutoff of 0.20; 99.2% (98.1-99.8%) using a cutoff of 0.15; 97.5% (95.8-98.7%) using a cutoff of 0.10; and 98.7% (97.3-99.5%) for the skin test. The estimated sensitivities were 8.3% (6.2-10.7%) for the AGID; 8.0% (6.0-10.4%), 10.6% (8.3-13.1%), and 16.3% (13.5-19.4%) for the ELISA using the cutoffs 0.20, 0.15, and 0.10 respectively; and 73.3% (62.3-85.8%) for the skin test. The skin test was specific in non-infected populations and sensitive in infected populations, although in some cases a positive skin test might represent MAP exposure rather than infection. The AGID and ELISA were specific but lacked sensitivity. The AGID and ELISA consistently identified two different populations of infected sheep with only moderate overlap between positive test results.