Evaluation of a thick and thin section method for estimation of podocyte number, glomerular volume, and glomerular volume per podocyte in rat kidney with Wilms' tumor-1 protein used as a podocyte nuclear marker

Podocyte loss and glomerular hypertrophy are associated with development of glomerulosclerosis, suggesting that there may be a maximal area for each podocyte in terms of its capacity to support and maintain the glomerular filter. This study hypothesized that exceeding this maximal threshold will res...

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Bibliographic Details
Published inJournal of the American Society of Nephrology Vol. 14; no. 10; pp. 2484 - 2493
Main Authors SANDEN, Silja K, WIGGINS, Jocelyn E, GOYAL, Meera, RIGGS, Lisa K, WIGGINS, Roger C
Format Journal Article
LanguageEnglish
Published Hagerstown, MD Lippincott Williams & Wilkins 01.10.2003
Subjects
Aging
Animals
Biological and medical sciences
Biomarkers
Carrier Proteins - metabolism
Cell Count - methods
Cell Nucleus - metabolism
DNA-Binding Proteins - metabolism
Kidney Cortex - cytology
Kidney Cortex - metabolism
Kidney Glomerulus - cytology
Kidney Glomerulus - metabolism
Kidneys
Medical sciences
Microtomy - methods
Nephrology. Urinary tract diseases
Nuclear Proteins - metabolism
Rats
Rats, Inbred BN
Tumors of the urinary system
Kidney disease
Urinary system disease
Rat
Rodentia
Exploration
Histology
Malignant tumor
Kidney
Podocyte
Anatomic pathology
Vertebrata
Mammalia
Animal
Wilms tumor
Technique
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Summary:Podocyte loss and glomerular hypertrophy are associated with development of glomerulosclerosis, suggesting that there may be a maximal area for each podocyte in terms of its capacity to support and maintain the glomerular filter. This study hypothesized that exceeding this maximal threshold will result in mesangial expansion and glomerulosclerosis. It may therefore be useful to measure podocyte number, glomerular volume, and glomerular volume per podocyte in clinical biopsy samples. An approach that uses thick and thin histologic sections cut from paraffin-embedded tissue to measure Wilms' tumor-1 protein-positive podocyte nuclear number and glomerular tuft area was studied. A rat model of aging has been used to track changes in glomerular podocyte number, glomerular volume per podocyte, and glomerular volume. Implications for clinical use of these variables are discussed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1046-6673
1533-3450
DOI:10.1097/01.ASN.0000089829.45296.7C