Antioxidant and L-asparaginase activities of culturable endophytic fungi from ornamental Dendrobium orchids

This study reports the antioxidant potential and L-asparaginase production of culturable fungal endophytes from Dendrobium orchids in Malaysia. Twenty-nine isolates were screened using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay to determine their free radical scavenging activities and antioxidan...

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Published inLetters in applied microbiology Vol. 77; no. 3
Main Authors Chua, Ru Wei, Song, Keang Peng, Ting, Adeline Su Yien
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.03.2024
Subjects
Antineoplastic Agents
Antineoplastic drugs
Antioxidants
Antioxidants - pharmacology
Asparaginase
Bioactive compounds
Dendrobium
Endophytes
Enzymatic activity
Free radicals
Fungi
Fusarium
Fusarium fujikuroi
L-asparaginase
Scavenging
orchids
bioactive compounds
antioxidant
L-asparaginase
fungal endophytes
Dendrobium
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ISSN1472-765X
0266-8254
1472-765X
DOI10.1093/lambio/ovad096

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Summary:This study reports the antioxidant potential and L-asparaginase production of culturable fungal endophytes from Dendrobium orchids in Malaysia. Twenty-nine isolates were screened using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay to determine their free radical scavenging activities and antioxidant capacity (IC50 and AEAC). L-asparaginase production of fungal endophytes was detected by the qualitative plate assay, and the enzyme activities estimated via the Nesslerization method. All 29 endophytic isolates exhibited various degrees of radical scavenging activities (35.37%–77.23%), with Fusarium fujikuroi (D1) identified as having the highest antioxidant capacity (IC50 6.097 mg/mL) and the highest AEAC value (11.55  mg/g). For L-asparaginase production, the majority of the isolates (89.66%) showed positive results, especially among the culturable species of Fusarium, Trichoderma, and Daldinia. Most Fusarium spp. were able to produce L-asparaginase (80.77%), but the highest L-asparaginase activity was detected in Daldinia eschscholtzii (D14) with 2.128 units/mL. Results from this study highlighted the potential of endophytic fungi from medicinal orchids (Dendrobium sp.) as natural sources of bioactive compounds to be developed into novel antioxidants and anticancer drugs.
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ISSN:1472-765X
0266-8254
1472-765X
DOI:10.1093/lambio/ovad096