GABA transporter 1 transcriptional starting site exhibiting tissue specific difference

GABA transporter 1(GAT1) takes important roles in multiple physiological processes through the uptake and release of GABA, but the regulation of GAT1 gene expression in different tissues is rarely known. To address the question, first, 5' Rapid amplification of cDNA end (RACE) was used to deter...

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Bibliographic Details
Published inCell research Vol. 11; no. 2; pp. 161 - 163
Main Authors Jin, X P, Huang, F, Yang, N, Lu, B F, Fei, J, Guo, L H
Format Journal Article
LanguageEnglish
Published England Nature Publishing Group 01.06.2001
The two authors contributed equally to the research.%Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences
Subjects
Aging - genetics
Animals
Animals, Newborn
Base Sequence - physiology
Brain - growth & development
Brain - metabolism
Carrier Proteins - genetics
Deoxyribonucleic acid
DNA
DNA, Complementary - analysis
DNA, Complementary - genetics
DNA, Complementary - metabolism
Exons - genetics
GABA Plasma Membrane Transport Proteins
Gene Expression Regulation - genetics
Intestines - growth & development
Intestines - metabolism
Introns - genetics
Male
Membrane Proteins - genetics
Membrane Transport Proteins
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Nucleic Acid Amplification Techniques - methods
Organic Anion Transporters
Physiology
Testis - growth & development
Testis - metabolism
Transcription, Genetic - genetics
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Summary:GABA transporter 1(GAT1) takes important roles in multiple physiological processes through the uptake and release of GABA, but the regulation of GAT1 gene expression in different tissues is rarely known. To address the question, first, 5' Rapid amplification of cDNA end (RACE) was used to determine GAT1 transcriptional starting sites in neonatal mouse cerebral cortex and intestine, adult mouse brain and adult rat testis. The products of 5'RACE were confirmed by DNA sequencing. We found that the transcript of GAT1 in neonatal mouse cerebral cortex and adult mouse brain starts at the same site (inside of exon 1), while in mouse intestine, GAT1 starts transcription in intron 1, and in rat testis, the transcript of GAT1 has an additional untranslation exon to the 5' direction.
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ISSN:1001-0602
1748-7838
DOI:10.1038/sj.cr.7290082