Antioxidant and Cytotoxic Activities of Aphanes arvensis Extracts

• Published in: Plant foods for human nutrition (Dordrecht) Vol. 65; no. 1; pp. 44 - 49
• Main Authors: Hamad, İsmail, Erol-Dayi, Özlem, Pekmez, Murat, Önay-Uçar, Evren, Arda, Nazlı
• Format: Journal Article
• Language: English
• Published: Boston Boston : Springer US 01.03.2010; Springer US; Springer
• Subjects: Antioxidants - adverse effects; Antioxidants - pharmacology; Ascorbic Acid; Biological and medical sciences; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Chromans; Cytotoxins; DNA - metabolism; DNA Damage; Dose-Response Relationship, Drug; Ecology; Food additives; Food industries; Food Science; Free Radical Scavengers - pharmacology; Fundamental and applied biological sciences. Psychology; General aspects; Inhibitory Concentration 50; Lipid Peroxidation - drug effects; Liposomes; Nutrition; Original Paper; Oxidation-Reduction; Plant Extracts - adverse effects; Plant Extracts - pharmacology; Plant Physiology; Protein Carbonylation - drug effects; Rosaceae; Methanol & water extracts; Cytotoxicity; Antioxidant and cytotoxic properties; Salad plants; Reactive oxygen species; Antioxidant activity; Aphanes arvensis; Vitamin; Liposome; Lipids; Extract; Method; Antioxidant; Protein; Free radical; Plasmid; DNA; Oxidation; Peroxidation
• ISSN: 0921-9668; 1573-9104
• DOI: 10.1007/s11130-009-0151-y

The objectives of this study were to examine the free radical scavenging activity and the protective effects against macromolecular oxidation as well as the cytotoxic activity of Aphanes arvensis aqueous and methanolic extracts. Free radical scavenging activity was determined by DPPH method. The methanolic extract showed a scavenging activity nearly equivalent to Trolox and Vitamin C and has an IC₅₀ value of 4.54 µg/mL. Total antioxidant capacity was determined by CUPRAC method. The antioxidant capacity of aqueous and methanolic extract was 0.792 and 1.550 mmol TE/g DWE, respectively. The protective effect of A. arvensis extracts against lipid peroxidation was evaluated using a liposome oxidation system. The methanolic extract was more active than the aqueous extract. The aqueous extract possessed protective effect against protein oxidation in a dose dependent manner. Both extracts showed inhibitory effect on DNA oxidation as measured by plasmid relaxation assay. Results presented here indicate that A. arvensis possess strong antioxidant activity and protective effects with very little cytotoxic effect, and they can therefore be used as a natural additive in food, cosmetic and pharmaceutical industries.