Production of hydrophilic phytotoxins by Mycosphaerella fijiensis

We have established a reproducible strategy to purify hydrophilic phytotoxins present in the aqueous filtrate of Mycosphaerella fijiensis Morelet. The lyophilized culture filtrate is initially treated with activated charcoal, then successively purified using vacuum liquid chromatography and semiprep...

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Bibliographic Details
Published inJournal of general plant pathology : JGPP Vol. 75; no. 3; pp. 191 - 195
Main Authors Cruz-Cruz, C.A.(Centro de Investigacion Cientifica de Yucatan (Mexico)), Garcia-Sosa, K, Escalante-Erosa, F, Pena-Rodriguez, L.M
Format Journal Article
LanguageEnglish
Published Japan Springer Japan 01.06.2009
Springer Nature B.V
Subjects
Agriculture
ASCOMYCOTINA
BANANAS
BANANE
BANANO
BIOASSAYS
Biomedical and Life Sciences
CHROMATOGRAPHIE
CHROMATOGRAPHY
CROMATOGRAFIA
Crop diseases
ENSAYO BIOLOGICO
FITOTOXINAS
Fungal Diseases
Life Sciences
Microbiology
Molecular biology
Musa
MYCOSPHAERELLA FIJIENSIS
Pathogens
PHYTOTOXINE
PHYTOTOXINS
Plant Pathology
PURIFICACION
PURIFICATION
TEST BIOLOGIQUE
Toxins
Black sigatoka
Leaf-spot assay
Bananas
Phytotoxins
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Summary:We have established a reproducible strategy to purify hydrophilic phytotoxins present in the aqueous filtrate of Mycosphaerella fijiensis Morelet. The lyophilized culture filtrate is initially treated with activated charcoal, then successively purified using vacuum liquid chromatography and semipreparative high performance liquid chromatography. Phytotoxic activity was tested using a leaf-spot assay on healthy banana leaves.
Bibliography:2009004698
H20
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ISSN:1345-2630
1610-739X
DOI:10.1007/s10327-009-0165-1